The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
A nucleoprotein complex mediates the integration of retroviral DNA.
Open accessAbstract
The integration of viral DNA into the host genome is an essential step in the retrovirus life cycle. To understand this process better, we have examined the native state of viral DNA in cells acutely infected by murine leukemia virus (MLV), using both a physical assay for viral DNA and a functional assay for integration activity (Brown et al. 1987). The viral DNA and integration activity copurify during velocity sedimentation, gel filtration, and density equilibrium centrifugation, indicating that viral DNA is in a large (approximately 160S) nucleoprotein complex that includes all functions required for integration activity in vitro. Analysis by immunoprecipitation shows that the viral capsid protein is part of the active nucleoprotein complex, but recognition of the complex by only a subset of anti-capsid sera implies that the protein is constrained conformationally. The viral DNA within this structure is accessible to nucleases; the effects of nucleases on the integrity of the complex suggest that the integration-competent particle is derived from and similar to the core of extracellular virions.
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Cites
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Self-citationChromatin Structure: Deduced from a Minichromosome
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Cited by
Integration of murine leukemia virus DNA depends on mitosis.
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Self-citationHuman immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B
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HIV-1 DNA integration: Mechanism of viral DNA cleavage and DNA strand transfer
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Active nuclear import of human immunodeficiency virus type 1 preintegration complexes.
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Metadata
- Authors
- Bowerman, B., Brown, P. O., Bishop, J. M., & Varmus, H. E.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1989-04
- Volume
- 3
- Issue
- 4
- Pages
- 469-478
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2135767314
- Cited by
- 377
- References
- 31
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Bowerman, B., Brown, P. O., Bishop, J. M., & Varmus, H. E. (1989). A nucleoprotein complex mediates the integration of retroviral DNA. Genes & Development. https://doi.org/10.1101/gad.3.4.469
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
