The DOI matches its Crossref record; 94 of the 94 references it cites were found in a registry or an index.
Differential regulation of E2F transactivation by cyclin/cdk2 complexes.
Open accessAbstract
The mammalian transcription factor E2F plays a critical role in the expression of genes required for cellular proliferation. To understand how E2F is regulated, we have developed a reconstituted in vitro transcription assay. Using this E2F-responsive assay, we can demonstrate that E2F-mediated transcription can be directly repressed by the tumor suppressor protein pRB. This inhibition is abolished by phosphorylation of pRB with either cyclin A/cdk2 or cyclin E/cdk2. However, these cyclin/kinase complexes exhibit differences in the ability to phosphorylate E2F. Only cyclin A/cdk2 can phosphorylate E2F effectively, and this phosphorylation abolishes its ability to bind DNA and mediate trans-activation. Thus, this in vitro transcriptional assay allows activation and inactivation of E2F transcription, and our findings demonstrate how transcriptional regulation of E2F can be linked to cell cycle-dependent activation of kinases.
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Cites
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Self-citation
Cited by
Sherr, C. J. (1996) · Science · doi:10.1126/science.274.5293.1672 · Cited by 5,379
The retinoblastoma protein and cell cycle control
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The regulation of E2F by pRB-family proteins.
Dyson, N. (1998) · Genes & Development · doi:10.1101/gad.12.15.2245 · Cited by 2,317
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Metadata
- Authors
- Dynlacht, B. D., Flores, O., Lees, J. A., & Harlow, E.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1994-08-01
- Volume
- 8
- Issue
- 15
- Pages
- 1772-1786
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2038765811
- Cited by
- 395
- References
- 94
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Dynlacht, B. D., Flores, O., Lees, J. A., & Harlow, E. (1994). Differential regulation of E2F transactivation by cyclin/cdk2 complexes. Genes & Development. https://doi.org/10.1101/gad.8.15.1772
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
