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Cited by 455

The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.

A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Matα2 repressor degradation

Open access
Swanson, Robert, Locher, Martin, Hochstrasser, Mark·2001·Genes & Development
DOI 10.1101/gad.933301
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Abstract

Substrate discrimination in the ubiquitin–proteasome system is believed to be dictated by specific combinations of ubiquitin–protein ligases (E3s) and ubiquitin-conjugating enzymes (E2s). Here we identify Doa10/Ssm4 as a yeast E3 that is embedded in the endoplasmic reticulum (ER)/nuclear envelope yet can target the soluble transcription factor Matα2. Doa10 contains an unusual RING finger, which has ubiquitin-ligase activity in vitro and is essential in vivo for degradation of α2 via its Deg1 degradation signal. Doa10 functions with two E2s, Ubc6 and Ubc7, to ubiquitinate Deg1 -bearing substrates, and it is also required for the degradation of at least one ER membrane protein. Interestingly, different short-lived ER proteins show distinct requirements for Doa10 and another ER-localized E3, Hrd1. Nevertheless, the two E3s overlap in function: A doa10 Δ hrd1 Δ mutant is far more sensitive to cadmium relative to either single mutant and displays strong constitutive induction of the unfolded protein response; this suggests a role for both E3s in eliminating aberrant ER proteins. The likely human ortholog of DOA10 is in the cri-du-chat syndrome critical region on chromosome 5p, suggesting that defective ubiquitin ligation might contribute to this common genetic disorder.

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Metadata

Authors
Swanson, R., Locher, M., & Hochstrasser, M.
Venue
Genes & Development
Publisher
Cold Spring Harbor Laboratory
Published
2001-10-15
Volume
15
Issue
20
Pages
2660-2674
ISSN
  • 0890-9369 (ISSN-L)
  • 1549-5477
DOI
10.1101/gad.933301
OpenAlex
W2146183177
Cited by
455
References
43
Type
posted content
Language
en
Open access
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CoChat. (2026). Citation check: A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Matα2 repressor degradation. https://app.cochat.ai/papers/10.1101/gad.933301
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Swanson, R., Locher, M., & Hochstrasser, M. (2001). A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Matα2 repressor degradation. Genes & Development. https://doi.org/10.1101/gad.933301

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