The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
DNA Mapping Using Microfluidic Stretching and Single-Molecule Detection of Fluorescent Site-Specific Tags
Open accessAbstract
We have developed a rapid molecular mapping technology—Direct Linear Analysis (DLA)—on the basis of the analysis of individual DNA molecules bound with sequence-specific fluorescent tags. The apparatus includes a microfluidic device for stretching DNA molecules in elongational flow that is coupled to a multicolor detection system capable of single-fluorophore sensitivity. Double-stranded DNA molecules were tagged at sequence-specific motif sites with fluorescent bisPNA (Peptide Nucleic Acid) tags. The DNA molecules were then stretched in the microfluidic device and driven in a flow stream past confocal fluorescence detectors. DLA provided the spatial locations of multiple specific sequence motifs along individual DNA molecules, and thousands of individual molecules could be analyzed per minute. We validated this technology using the 48.5 kb λ phage genome with different 8-base and 7-base sequence motif tags. The distance between the sequence motifs was determined with an accuracy of ±0.8 kb, and these tags could be localized on the DNA with an accuracy of ±2 kb. Thus, DLA is a rapid mapping technology, suitable for analysis of long DNA molecules.
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Cites
The Structure of Haplotype Blocks in the Human Genome
Gabriel, S. B., Schaffner, S. F., Nguyen, H., Moore, J. M., Roy, J., Blumenstiel, B., Higgins, J., DeFelice, M., Lochner, A., Faggart, M., et al. (2002) · Science · doi:10.1126/science.1069424 · Cited by 5,863
Blocks of Limited Haplotype Diversity Revealed by High-Resolution Scanning of Human Chromosome 21
Patil, N., Berno, A. J., Hinds, D. A., Barrett, W. A., Doshi, J. M., Hacker, C. R., Kautzer, C. R., Lee, D. H., Marjoribanks, C., McDonough, D. P., et al. (2001) · Science · doi:10.1126/science.1065573 · Cited by 1,145
Cited by
A single-molecule method for the quantitation of microRNA gene expression
Neely, L. A., Patel, S., Garver, J., Gallo, M., Hackett, M., McLaughlin, S., Nadel, M., Harris, J., Gullans, S., & Rooke, J. (2006) · Nature Methods · doi:10.1038/nmeth825 · Cited by 284
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Metadata
- Authors
- Chan, E. Y., Goncalves, N. M., Haeusler, R. A., Hatch, A. J., Larson, J. W., Maletta, A. M., Yantz, G. R., Carstea, E. D., Fuchs, M., Wong, G. G., Gullans, S. R., & Gilmanshin, R.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2004-06
- Volume
- 14
- Issue
- 6
- Pages
- 1137-1146
- ISSN
- 1088-9051 (ISSN-L)
- OpenAlex
- W2131378632
- Cited by
- 165
- References
- 2
- Type
- posted content
- Language
- en
- Open access
- bronze
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- Chan, E. Y., Goncalves, N. M., Haeusler, R. A., Hatch, A. J., Larson, J. W., Maletta, A. M., Yantz, G. R., Carstea, E. D., Fuchs, M., Wong, G. G., Gullans, S. R., & Gilmanshin, R. (2004). DNA Mapping Using Microfluidic Stretching and Single-Molecule Detection of Fluorescent Site-Specific Tags. Genome Research. https://doi.org/10.1101/gr.1635204
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