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Cited by 282

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome

Open access
Osoegawa, Kazutoyo, Mammoser, Aaron G., Wu, Chenyan, Frengen, Eirik, Zeng, Changjiang, Catanese, Joseph J., de Jong, Pieter J.·2001·Genome Research

Abstract

A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large average insert size (178 kb) has been constructed to provide the intermediate substrate for the international genome sequencing effort. The DNA was obtained from a single anonymous volunteer, whose identity was protected through a double-blind donor selection protocol. DNA fragments were generated by partial digestion with Eco RI (library segments 1–4: 24-fold) and Mbo I (segment 5: sixfold) and cloned into the pBACe3.6 and pTARBAC1 vectors, respectively. The quality of the library was assessed by extensive analysis of 169 clones for rearrangements and artifacts. Eighteen BACs (11%) revealed minor insert rearrangements, and none was chimeric. This BAC library, designated as “RPCI-11,” has been used widely as the central resource for insert-end sequencing, clone fingerprinting, high-throughput sequence analysis and as a source of mapped clones for diagnostic and functional studies. The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AQ936150–AQ936491.]

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Metadata

Authors
Osoegawa, K., Mammoser, A. G., Wu, C., Frengen, E., Zeng, C., Catanese, J. J., & de Jong, P. J.
Venue
Genome Research
Published
2001-03-01
Volume
11
Issue
3
Pages
483-496
ISSN
  • 1088-9051 (ISSN-L)
  • 1549-5469
OpenAlex
W2026652568
Cited by
282
References
1
Type
posted content
Language
en
Open access
bronze

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CoChat. (2026). Citation check: A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome. https://app.cochat.ai/papers/10.1101/gr.169601
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Osoegawa, K., Mammoser, A. G., Wu, C., Frengen, E., Zeng, C., Catanese, J. J., & de Jong, P. J. (2001). A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome. Genome Research. https://doi.org/10.1101/gr.169601

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