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Cited by 688

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.

Phenotype MicroArrays for High-Throughput Phenotypic Testing and Assay of Gene Function

Open access
Bochner, Barry R., Gadzinski, Peter, Panomitros, Eugenia·2001·Genome Research
DOI 10.1101/gr.186501
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Abstract

The bacterium Escherichia coli is used as a model cellular system to test and validate a new technology called Phenotype MicroArrays (PMs). PM technology is a high-throughput technology for simultaneous testing of a large number of cellular phenotypes. It consists of preconfigured well arrays in which each well tests a different cellular phenotype and an automated instrument that continuously monitors and records the response of the cells in all wells of the arrays. For example, nearly 700 phenotypes of E. coli can be assayed by merely pipetting a cell suspension into seven microplate arrays. PMs can be used to directly assay the effects of genetic changes on cells, especially gene knock-outs. Here, we provide data on phenotypic analysis of six strains and show that we can detect expected phenotypes as well as, in some cases, unexpected phenotypes.

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Metadata

Authors
Bochner, B. R., Gadzinski, P., & Panomitros, E.
Venue
Genome Research
Publisher
Cold Spring Harbor Laboratory
Published
2001-07-01
Volume
11
Issue
7
Pages
1246-1255
ISSN
  • 1088-9051 (ISSN-L)
  • 1549-5469
DOI
10.1101/gr.186501
OpenAlex
W1984852783
Cited by
688
References
24
Type
posted content
Language
en
Open access
bronze

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