The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Fast and accurate de novo genome assembly from long uncorrected reads
Open accessAbstract
The assembly of long reads from Pacific Biosciences and Oxford Nanopore Technologies typically requires resource-intensive error-correction and consensus-generation steps to obtain high-quality assemblies. We show that the error-correction step can be omitted and that high-quality consensus sequences can be generated efficiently with a SIMD-accelerated, partial-order alignment–based, stand-alone consensus module called Racon. Based on tests with PacBio and Oxford Nanopore data sets, we show that Racon coupled with miniasm enables consensus genomes with similar or better quality than state-of-the-art methods while being an order of magnitude faster.
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Cites
Real-time selective sequencing using nanopore technology
Loose, M., Malla, S., & Stout, M. (2016) · Nature Methods · doi:10.1038/nmeth.3930 · Cited by 399
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Wick, R. R., Judd, L. M., & Holt, K. E. (2019) · Genome Biology · doi:10.1186/s13059-019-1727-y · Cited by 3,221
The complete sequence of a human genome
Nurk, S., Koren, S., Rhie, A., Rautiainen, M., Bzikadze, A. V., Mikheenko, A., Vollger, M. R., Altemose, N., Uralsky, L., Gershman, A., et al. (2022) · Science · doi:10.1126/science.abj6987 · Cited by 3,136
Self-citationOpportunities and challenges in long-read sequencing data analysis
Amarasinghe, S. L., Su, S., Dong, X., Zappia, L., Ritchie, M. E., & Gouil, Q. (2020) · Genome Biology · doi:10.1186/s13059-020-1935-5 · Cited by 2,530
Next-Generation Sequencing Technology: Current Trends and Advancements
Satam, H., Joshi, K., Mangrolia, U., Waghoo, S., Zaidi, G., Rawool, S., Thakare, R. P., Banday, S., Mishra, A. K., Das, G., et al. (2023) · Biology · doi:10.3390/biology12070997 · Cited by 1,299
De novo assembly, annotation, and comparative analysis of 26 diverse maize genomes
Hufford, M. B., Seetharam, A. S., Woodhouse, M. R., Chougule, K. M., Ou, S., Liu, J., Ricci, W. A., Guo, T., Olson, A., Qiu, Y., et al. (2021) · Science · doi:10.1126/science.abg5289 · Cited by 747
Metadata
- Authors
- Vaser, R., Sović, I., Nagarajan, N., & Šikić, M.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2017-01-18
- Volume
- 27
- Issue
- 5
- Pages
- 737-746
- ISSN
- 1088-9051 (ISSN-L)
- 1549-5469
- OpenAlex
- W2575750030
- Cited by
- 3,043
- References
- 1
- Type
- posted content
- Language
- en
- Open access
- bronze
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- Vaser, R., Sović, I., Nagarajan, N., & Šikić, M. (2017). Fast and accurate de novo genome assembly from long uncorrected reads. Genome Research. https://doi.org/10.1101/gr.214270.116
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