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A Microarray-Based Antibiotic Screen Identifies a Regulatory Role for Supercoiling in the Osmotic Stress Response of Escherichia coli
Open accessAbstract
Changes in DNA supercoiling are induced by a wide range of environmental stresses in Escherichia coli , but the physiological significance of these responses remains unclear. We now demonstrate that an increase in negative supercoiling is necessary for transcriptional activation of a large subset of osmotic stress-response genes. Using a microarray-based approach, we have characterized supercoiling-dependent gene transcription by expression profiling under conditions of high salt, in conjunction with the microbial antibiotics novobiocin, pefloxacin, and chloramphenicol. Algorithmic clustering and statistical measures for gauging cellular function show that this subset is enriched for genes critical in osmoprotectant transport/synthesis and rpoS -driven stationary phase adaptation. Transcription factor binding site analysis also supports regulation by the global stress ς factor rpoS . In addition, these studies implicate 60 uncharacterized genes in the osmotic stress regulon, and offer evidence for a broader role for supercoiling in the control of stress-induced transcription.
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Cites
Responses of E. coli to osmotic stress: large changes in amounts of cytoplasmic solutes and water
Record Jr, M. T., Courtenay, E. S., Cayley, D. S., & Guttman, H. J. (1998) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(98)01196-7 · Cited by 357
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Mitchell, A., Romano, G. H., Groisman, B., Yona, A., Dekel, E., Kupiec, M., Dahan, O., & Pilpel, Y. (2009) · Nature · doi:10.1038/nature08112 · Cited by 581
Predictive Behavior Within Microbial Genetic Networks
Tagkopoulos, I., Liu, Y.-C., & Tavazoie, S. (2008) · Science · doi:10.1126/science.1154456 · Cited by 519
Powell, A. L. T., Nguyen, C. V., Hill, T., Cheng, K. L., Figueroa-Balderas, R., Aktas, H., Ashrafi, H., Pons, C., Fernández-Muñoz, R., Vicente, A., et al. (2012) · Science · doi:10.1126/science.1222218 · Cited by 481
Mechanisms of survival, responses and sources of Salmonella in low-moisture environments
Finn, S., Condell, O., McClure, P., Amézquita, A., & Fanning, S. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00331 · Cited by 398
Dorman, C. J. (2007) · Nature Reviews Microbiology · doi:10.1038/nrmicro1598 · Cited by 362
Metadata
- Authors
- Cheung, K. J., Badarinarayana, V., Selinger, D. W., Janse, D., & Church, G. M.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2003-01-14
- Volume
- 13
- Issue
- 2
- Pages
- 206-215
- ISSN
- 1088-9051 (ISSN-L)
- 1549-5469
- OpenAlex
- W2010321216
- Cited by
- 170
- References
- 1
- Type
- posted content
- Language
- en
- Open access
- green
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- Cheung, K. J., Badarinarayana, V., Selinger, D. W., Janse, D., & Church, G. M. (2003). A Microarray-Based Antibiotic Screen Identifies a Regulatory Role for Supercoiling in the Osmotic Stress Response of Escherichia coli. Genome Research. https://doi.org/10.1101/gr.401003
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