Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function
Open accessAbstract
Deep mutational scanning is a method that makes use of next-generation sequencing technology to measure in a single experiment the activity of 10 5 or more unique variants of a protein. Because of this depth of mutational coverage, this strategy provides data that can be analyzed to reveal many protein properties. Deep mutational scanning approaches are particularly amenable to being performed in Saccharomyces cerevisiae, given the extensive toolkit of reagents and technologies available for this organism.
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References 15 · Cited by 32
- Authors
- Starita, L. M., & Fields, S.
- Affiliations
- University of Washington (US)
- Howard Hughes Medical Institute (US)
- Venue
- Cold Spring Harbor Protocols
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2015-08
- Volume
- 2015
- Issue
- 8
- Pages
- pdb.top077503
- ISSN
- 1559-6095 (ISSN-L)
- 1940-3402
- OpenAlex
- W1943048093
- Cited by
- 32
- References
- 15
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 2 · 2022: 6 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 5 · 2015: 1
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cold Spring Harbor Protocols (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Keywords
- deep mutational scanning, protein variants, protein function, mutation effects, next-generation sequencing, Saccharomyces cerevisiae, protein properties
- MeSH terms
- DNA Mutational Analysis, Genetic Testing, Molecular Biology, Saccharomyces cerevisiae, Sequence Analysis, DNA, High-Throughput Screening Assays, High-Throughput Nucleotide Sequencing
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