Enzymatic catalysis and the transition state theory of reaction rates: transition state analogs
Abstract
In 1948 Pauling gave the following qualitative description of enzymatic catalysis in terms of the activated complex or transition state theory of reaction rates: "... I believe that ... the surface configuration of the enzyme is ... complementary to an unstable molecule with only transient existence—namely, the 'activated complex' for the reaction that is catalyzed by the enzyme. The mode of action of an enzyme would then be the following: the enzyme would show a small power of attraction for the substrate molecule or molecules, which would become attached to it in its active surface region. This substrate molecule, or these molecules, would then be strained by the forces of attraction to the enzyme, which would tend to deform it into the configuration of the activated complex, for which the power of attraction by the enzyme is the greatest. The activated complex would then, under the influence of ordinary thermal...
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VRC was identified as having a significant impact on RNA quality.
“Among these, the vanadyl ribonucleoside complex (VRC) (Lienhard et al., 1972) was identified as having a significant impact on RNA quality.”
From Robust single-nucleus RNA sequencing reveals depot-specific cell population dynamics in adipose tissue remodeling during obesity (2025) · cites this paper as Lienhard et al., 1972
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So, J., Strobel, O., Wann, J., Kim, K., Paul, A., Acri, D. J., Dabin, L. C., Kim, J., Peng, G., & Roh, H. C. (2025) · eLife · doi:10.7554/elife.97981 · Cited by 18 · see citing works
- Authors
- Lienhard, G. E., Secemski, I. I., Koehler, K. A., & Lindquist, R. N.
- Affiliations
- Harvard University (US)
- ORCID
- Koehler, K. A.
- Venue
- Cold Spring Harbor Symposia on Quantitative Biology
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1972-01-01
- Volume
- 36
- Issue
- 0
- Pages
- 45-51
- ISSN
- 0091-7451 (ISSN-L)
- 1943-4456
- OpenAlex
- W2093673843
- Cited by
- 89
- References
- 0
- Citations by year
- 2025: 1 · 2024: 3 · 2022: 2 · 2020: 1 · 2017: 1 · 2015: 1 · 2012: 1
- Type
- posted content
- Language
- en
- Open access
- closed
- Locations
- Cold Spring Harbor Symposia on Quantitative Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Chemical Reaction Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme function and inhibition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- enzyme catalysis, transition state theory, transition state analogues, transition state, substrate binding, enzyme active site, reaction rate, Pauling
- MeSH terms
- Binding Sites, Boronic Acids, Catalysis, Chymotrypsin, Cinnamates, Deoxyuridine, Enzymes, Kinetics, Lactones, Mathematics, Muramidase, Pancreas, Protein Binding, Protein Conformation, Pyrimidine Nucleotides, Ribonucleases, Uracil Nucleotides, Vanadium
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