Mutations Defining the Operator-binding Sites of Bacteriophage Repressor
Abstract
We have characterized about 50 different amino acid substitutions in the aminoterminal domain of lambda repressor. Sixteen of these substitutions alter external side chains of the repressor and cause a substantial reduction in the affinity of the mutant repressor for operator DNA. Seven of these mutant repressors were purified and were shown to be stably folded. The strong, external repressor mutations occur near the aminoterminal end of alpha helix 2, throughout alpha helix 3, and in the aminoterminal-arm region of the repressor. These results suggest that these regions of lambda repressor are close to operator DNA in the protein-DNA complex and thus that these regions comprise the DNA-binding sites of the repressor. Our genetic results support and are completely consistent with more-detailed models of the repressor-operator interaction based on model-building (Pabo and Lewis 1982; Lewis et al. this volume) and biochemical studies (Pabo et al. 1982).
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QL44 and IS84 are mutations in the DNA-binding domain of λ repressor that act as dominant negative alleles in intact λ repressor.
“QL44 and IS84 are mutations in the DNA-binding domain of λ repressor that act as dominant negative alleles in intact λ repressor (Hecht et al., 1983; Nelson et al., 1983).”
From Oligomerization properties of GCN4 leucine zipper <i>e</i> and <i>g</i> position mutants (1997) · cites this paper as Nelson et al., 1983
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“repressor DNA-binding domain that destroy specific DNA binding have a dominant negative phenotype (Nelson et al., 1983).”
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- Authors
- Nelson, H. C. M., Hecht, M. H., & Sauer, R. T.
- Affiliations
- Massachusetts Institute of Technology (US)
- Venue
- Cold Spring Harbor Symposia on Quantitative Biology
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1983-01-01
- Volume
- 47
- Issue
- 0
- Pages
- 441-449
- ISSN
- 0091-7451 (ISSN-L)
- 1943-4456
- OpenAlex
- W2328887676
- Cited by
- 49
- References
- 0
- Citations by year
- 2020: 1 · 2016: 1 · 2013: 1
- Type
- posted content
- Language
- en
- Open access
- closed
- Locations
- Cold Spring Harbor Symposia on Quantitative Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- operator DNA, DNA binding sites, amino acid substitutions, repressor-operator interaction, protein-DNA complex
- MeSH terms
- Amino Acid Sequence, Base Sequence, DNA, Viral, Genes, Dominant, Genes, Viral, Genotype, Mutation, Operon, Bacteriophage lambda, Protein Binding, Repressor Proteins, Transcription Factors, Viral Proteins, Viral Regulatory and Accessory Proteins
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