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Fluorescence Fluctuation Spectroscopy in Subdiffraction Focal Volumes
Open accessAbstract
We establish fluorescence fluctuation spectroscopy (FFS) with nanoscale detection volumes generated by stimulated emission depletion. Our method applies fluorescence correlation spectroscopy and fluorescence intensity distribution analysis to extract molecular information about mobilities and fluorescence emission in solution. The combination of correlation analysis with that of photon intensity distributions reveals a fivefold squeezing of the detection volume over current diffraction-limited systems, which is in full agreement with the simultaneously demonstrated 25-fold reduction in (axial) focal transit time. Our method significantly extends the potential of far-field FFS, including for the noninvasive investigation of molecular reactions at higher concentrations.
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Citations
Cited by
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
Self-citationFluorescence correlation spectroscopy in living cells
Kim, S. A., Heinze, K. G., & Schwille, P. (2007) · Nature Methods · doi:10.1038/nmeth1104 · Cited by 444
Stimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 339
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Metadata
- Authors
- Kastrup, L., Blom, H., Eggeling, C., & Hell, S. W.
- Venue
- Physical Review Letters
- Publisher
- American Physical Society (APS)
- Published
- 2005-05-04
- Volume
- 94
- Issue
- 17
- Pages
- 178104-178104
- ISSN
- 0031-9007 (ISSN-L)
- 1079-7114
- OpenAlex
- W2022393754
- Cited by
- 229
- References
- 0
- FWCI
- 7.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Kastrup, L., Blom, H., Eggeling, C., & Hell, S. W. (2005). Fluorescence Fluctuation Spectroscopy in Subdiffraction Focal Volumes. Physical Review Letters. https://doi.org/10.1103/physrevlett.94.178104
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