The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
myo-Inositol Oxygenase Offers a Possible Entry Point into Plant Ascorbate Biosynthesis
Open accessAbstract
Two biosynthetic pathways for ascorbate (l-ascorbic acid [AsA]; vitamin C) in plants are presently known, the mannose/l-galactose pathway and an l-GalUA pathway. Here, we present molecular and biochemical evidence for a possible biosynthetic route using myo-inositol (MI) as the initial substrate. A MI oxygenase (MIOX) gene was identified in chromosome 4 (miox4) of Arabidopsis ecotype Columbia, and its enzymatic activity was confirmed in bacterially expressed recombinant protein. Miox4 was primarily expressed in flowers and leaves of wild-type Arabidopsis plants, tissues with a high concentration of AsA. Ascorbate levels increased 2- to 3-fold in homozygous Arabidopsis lines overexpressing the miox4 open reading frame, thus suggesting the role of MI in AsA biosynthesis and the potential for using this gene for the agronomic and nutritional enhancement of crops.
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Metadata
- Authors
- Lorence, A., Chevone, B. I., Mendes, P., & Nessler, C. L.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-03-01
- Volume
- 134
- Issue
- 3
- Pages
- 1200-1205
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2133156589
- Cited by
- 494
- References
- 29
- FWCI
- 6.45
- Type
- journal article
- Language
- en
- Open access
- green
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- Lorence, A., Chevone, B. I., Mendes, P., & Nessler, C. L. (2004). myo-Inositol Oxygenase Offers a Possible Entry Point into Plant Ascorbate Biosynthesis. Plant Physiology. https://doi.org/10.1104/pp.103.033936
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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