The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Arabidopsis Calcium-Dependent Protein Kinase AtCPK32 Interacts with ABF4, a Transcriptional Regulator of Abscisic Acid-Responsive Gene Expression, and Modulates Its Activity
Abstract
The phytohormone abscisic acid (ABA) regulates stress-responsive gene expression during vegetative growth. The ABA regulation of many genes is mediated by a subfamily of basic leucine zipper class transcription factors referred to as ABFs (i.e. ABF1–ABF4), whose transcriptional activity is induced by ABA. Here we show that a calcium-dependent protein kinase is involved in the ABA-dependent activation process. We carried out yeast two-hybrid screens to identify regulatory components of ABF4 function and isolated AtCPK32 as an ABF4-interacting protein. AtCPK32 has autophosphorylation activity and can phosphorylate ABF4 in vitro. Mutational analysis indicated that serine-110 of ABF4, which is highly conserved among ABF family members, may be phosphorylated by AtCPK32. The serine-110 residue is essential for ABF4-AtCPK32 interaction, and transient expression assay revealed that it is also required for the normal transcriptional function of ABF4. The expression patterns and subcellular localization of AtCPK32 are similar to those of ABF4. Furthermore, its overexpression affects both ABA sensitivity and the expression of a number of ABF4-regulated genes. Together, our data demonstrate that AtCPK32 is an ABA signaling component that regulates the ABA-responsive gene expression via ABF4.
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Cites
The Leucine Zipper: A Hypothetical Structure Common to a New Class of DNA Binding Proteins
Landschulz, W. H., Johnson, P. F., & McKnight, S. L. (1988) · Science · doi:10.1126/science.3289117 · Cited by 3,443
Kim, K.-N., Cheong, Y. H., Grant, J. J., Pandey, G. K., & Luan, S. (2003) · The Plant Cell · doi:10.1105/tpc.006858 · Cited by 406
Cited by
Molecular Aspects of Seed Dormancy
Finkelstein, R., Reeves, W., Ariizumi, T., & Steber, C. (2008) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.59.032607.092740 · Cited by 1,379
Research on plant abiotic stress responses in the post‐genome era: past, present and future
Hirayama, T., & Shinozaki, K. (2010) · The Plant Journal · doi:10.1111/j.1365-313x.2010.04124.x · Cited by 1,215
Zhu, S.-Y., Yu, X.-C., Wang, X.-J., Zhao, R., Li, Y., Fan, R.-C., Shang, Y., Du, S.-Y., Wang, X.-F., Wu, F.-Q., et al. (2007) · The Plant Cell · doi:10.1105/tpc.107.050666 · Cited by 632
Hirayama, T., & Shinozaki, K. (2007) · Trends in Plant Science · doi:10.1016/j.tplants.2007.06.013 · Cited by 494
Comparative physiology of elemental distributions in plants
Conn, S., & Gilliham, M. (2010) · Annals of Botany · doi:10.1093/aob/mcq027 · Cited by 344
Metadata
- Authors
- Choi, H.-I., Park, H.-J., Park, J. H., Kim, S., Im, M.-Y., Seo, H.-H., Kim, Y.-W., Hwang, I., & Kim, S. Y.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-11-18
- Volume
- 139
- Issue
- 4
- Pages
- 1750-1761
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2030978693
- Cited by
- 353
- References
- 2
- FWCI
- 8.41
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Choi, H.-I., Park, H.-J., Park, J. H., Kim, S., Im, M.-Y., Seo, H.-H., Kim, Y.-W., Hwang, I., & Kim, S. Y. (2005). Arabidopsis Calcium-Dependent Protein Kinase AtCPK32 Interacts with ABF4, a Transcriptional Regulator of Abscisic Acid-Responsive Gene Expression, and Modulates Its Activity. Plant Physiology. https://doi.org/10.1104/pp.105.069757
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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