CoChat
Sign Up
Cited by 296Plant Micronutrient Interactions and EffectsIron Metabolism and DisordersPlant nutrient uptake and metabolism

The DOI matches its Crossref record.

Iron Deficiency-Induced Secretion of Phenolics Facilitates the Reutilization of Root Apoplastic Iron in Red Clover

Open access
Jin, Chong Wei, You, Guang Yi, He, Yun Feng, Tang, Caixian, Wu, Ping, Zheng, Shao Jian·2007·Plant Physiology
DOI 10.1104/pp.107.095794PMC PMC1913808
Open this paper in CoChat — free account

Abstract

Phenolic compounds are frequently reported to be the main components of root exudates in response to iron (Fe) deficiency in Strategy I plants, but relatively little is known about their function. Here, we show that removal of secreted phenolics from the root-bathing solution almost completely inhibited the reutilization of apoplastic Fe in roots of red clover (Trifolium pratense). This resulted in much lower levels of shoot Fe and significantly higher root Fe compared with control and also resulted in leaf chlorosis, suggesting this approach stimulated Fe deficiency. This was supported by the observation that phenolic removal significantly enhanced root ferric chelate reductase activity, which is normally induced by plant Fe deficiency. Furthermore, root proton extrusion, which also is normally increased during Fe deficiency, was found to be higher in plants exposed to the phenolic removal treatment too. These results indicate that Fe deficiency-induced phenolics secretion plays an important role in the reutilization of root apoplastic Fe, and this reutilization is not mediated by proton extrusion or the root ferric chelate reductase. In vitro studies with extracted root cell walls further demonstrate that excreted phenolics efficiently desorbed a significant amount of Fe from cell walls, indicating a direct involvement of phenolics in Fe remobilization. All of these results constitute the first direct experimental evidence, to our knowledge, that Fe deficiency-induced secretion of phenolics by the roots of a dicot species improves plant Fe nutrition by enhancing reutilization of apoplastic Fe, thereby improving Fe nutrition in the shoot.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1104/pp.107.095794

Cites

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Chemical removal of excreted phenolic acids from the nutrient solution of red clover results in iron deficiency in red clover.

    “Chemical removal of excreted phenolic acids from the nutrient solution of red clover (Trifolium pretense) was found to result in iron deficiency in red clover (Jin et al., 2007).”

    From Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional Trichotomy (2020) · cites this paper as Jin et al., 2007

    The Discussion reports that removing secreted phenolics made iron-deficiency symptoms more severe in red clover already grown under iron-deficient conditions. It also says the treatment did not affect chlorophyll or growth in iron-sufficient plants. The claim overstates the result by saying removal resulted in iron deficiency, rather than exacerbating an existing deficiency. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 33

  1. Azia F, Stewart KA (2001) Relationships between extractable chlorophyll and SPAD values in muskmelon leaves. J Plant Nutr 24 961–966 [Google Scholar]
  2. Bienfait HF, Van den Briel W, Mesland-Mul NT (1985) Free space iron pools in roots: generation and mobilization. Plant Physiol 78 596–600 [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blum U, Staman KL, Flint LJ, Shafer SR (2000) Induction and/or selection of phenolic acid-utilizing bulk-soil and rhizosphere bacteria and their influence on phenolic acid phytotoxicity. J Chem Ecol 26 2059–2078 [Google Scholar]
  4. Cao G, Sofic E, Prior RL (1997) Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radic Biol Med 22 749–760 [DOI] [PubMed] [Google Scholar]
  5. Chen Y, Shi J, Tian G, Zheng S, Lin Q (2004) Fe deficiency induces Cu uptake and accumulation in Commelina communis. Plant Sci 166 1371–1377 [Google Scholar]
  6. Cohen CK, Fox TC, Garvin DF, Kochian LV (1998) The role of iron-deficiency stress responses in stimulating heavy-metal transport in plants. Plant Physiol 116 1063–1072 [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Curie C, Briat JF (2003) Iron transport and signaling in plants. Annu Rev Plant Biol 54 183–206 [DOI] [PubMed] [Google Scholar]
  8. Dakora FD, Phillips DA (2002) Root exudates as mediators of mineral acquisition in low-nutrient environments. Plant Soil 245 35–47 [Google Scholar]
  9. Grusak MA (1995) Whole-root iron(III)-reductase activity throughout the life cycle of iron-grown Pisum sativum L. (Fabaceae): relevance to the iron nutrition of developing seeds. Planta 197 111–117 [Google Scholar]
  10. Guerinot ML, Yi Y (1994) Iron: nutritious, noxious, and not readily available. Plant Physiol 104 815–820 [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hell R, Stephan UW (2003) Iron uptake, trafficking and homeostasis in plants. Planta 216 541–551 [DOI] [PubMed] [Google Scholar]
  12. Imsande J (1998) Iron, sulfur, and chlorophyll deficiencies: a need for an integrative approach in plant physiology. Physiol Plant 103 139–144 [Google Scholar]
  13. Jauregui MA, Reisenauer HM (1982) Calcium carbonate and manganese dioxide as regulators of available manganese and iron. Soil Sci 134 105–110 [Google Scholar]
  14. Jin CW, He YF, Tang CX, Wu P, Zheng SJ (2006) Mechanisms of microbially enhanced Fe acquisition in red clover (Trifolium pretense L.). Plant Cell Environ 29 888–897 [DOI] [PubMed] [Google Scholar]
  15. Kim SA, Punshon T, Lanzirott A, Li L, Alonso JM, Ecker JR, Kaplan J, Guerinot ML (2006) Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1. Science 314 1295–1298 [DOI] [PubMed] [Google Scholar]
  16. Longnecker N (1986) A comparison resistance of soybean and sunflower to iron-deficiency induced chlorosis. PhD thesis. Cornell University, Ithaca, NY
  17. Longnecker N, Welch RM (1990) Accumulation of apoplastic iron in plant roots: a factor in the resistance of soybeans to iron-deficiency induced chlorosis? Plant Physiol 92 17–22 [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lynch JM, Whipps JM (1990) Substrate flow in the rhizosphere. Plant Soil 129 1–10 [Google Scholar]
  19. Marschner H (1995) Mineral Nutrition of Higher Plant. Academic Press, London
  20. Ohwaki Y, Sugahara K (1997) Active extrusion of protons and exudation of carboxylic acids in response to iron deficiency by roots of chickpea (Cicer arietnum L.). Plant Soil 189 49–55 [Google Scholar]
  21. Rice-Evans CA, Miller NJ (1996) Antioxidant activities of flavonoids as bioactive components of food. Biochem Soc Trans 24 790–795 [DOI] [PubMed] [Google Scholar]
  22. Robinson NJ, Procter CM, Connolly EL, Guerinot ML (1999) A ferric-chelate reductase for iron uptake from soils. Nature 397 694–697 [DOI] [PubMed] [Google Scholar]
  23. Römheld V, Maschner H (1986) Mobilization of iron in the rhizosphere of different plant species. Adv Plant Nutr 2 155–204 [Google Scholar]
  24. Singleton VL, Rossi JAJ (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16 144–158 [Google Scholar]
  25. Susín S, Abián J, Sánchez-Beyes JA, Peleato ML, Abadia A, Gelpi E, Abadia J (1996) Riboflavin 3′- and 5′-sulphate, two novel flavins accumulating in the roots of iron-deficient sugar beet (Beta vulgaris). J Biol Chem 268 20958–20965 [PubMed] [Google Scholar]
  26. Takagi S (1976) Naturally occurring iron-chelating compounds in oat- and rice-root washing. I. Activity measurement and preliminary characterization. Soil Sci Plant Nutr 22 423–433 [Google Scholar]
  27. Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J (2006) A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 134 1298–1300 [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vose PB (1982) Iron nutrition in plants: a world overview. J Plant Nutr 5 233–249 [Google Scholar]
  29. Welch RM, Norvell WA (1993) Growth and nutrient uptake by barley (Hordeum vulgare L. cv Herta): studies using an N-(2-hydroxyethyl)ethylenedinitrilotriacetic acid-buffered nutrient solution technique. II. Role of zinc in the uptake and root leakage of mineral nutrients. Plant Physiol 101 627–631 [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Welkie GW (2000) Taxonomic distribution of dicotyledonous species capable of root excretion of riboflavin under iron deficiency. J Plant Nutr 23 1819–1831 [Google Scholar]
  31. Zhang FS, Romheld V, Marschner H (1991) Role of the root apoplasm for iron acquisition by wheat plants. Plant Physiol 97 1302–1305 [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Zheng SJ, Tang CX, Arakawa Y, Masaoka Y (2003) The responses of red clover (Trifolium pratense L.) to iron deficiency: a root Fe(III) chelate reductase. Plant Sci 164 679–687 [Google Scholar]
  33. Zhong H, Läuchli A (1993) Changes of the cell wall composition and polymer in primary roots of cotton seedlings under salinity. J Exp Bot 44 773–778 [Google Scholar]

Cited by

Sorted by citations

Metadata

Authors
Jin, C. W., You, G. Y., He, Y. F., Tang, C., Wu, P., & Zheng, S. J.
Affiliations
La Trobe University (AU)
ORCID
Venue
Plant Physiology
Publisher
Oxford University Press (OUP)
Published
2007-03-16
Volume
144
Issue
1
Pages
278-285
ISSN
  • 0032-0889 (ISSN-L)
  • 1532-2548
DOI
10.1104/pp.107.095794
PMC
PMC1913808
OpenAlex
W2119750302
Cited by
296
References
0
Citations by year
2026: 10 · 2025: 15 · 2024: 24 · 2023: 21 · 2022: 18 · 2021: 21 · 2020: 26 · 2019: 13 · 2018: 16 · 2017: 15 · 2016: 20 · 2015: 17 · 2014: 19 · 2013: 18 · 2012: 10
FWCI
8.91
Type
journal article
Language
en
Open access
Licence
aspb.org/publications/aspb-journals/open-articles (vor, 412 d)
Locations
  • PLANT PHYSIOLOGY (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China
  • Program for New Century Excellent Talents in University
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
Keywords
iron deficiency, phenolic compounds, root exudates, iron remobilization, red clover, Strategy I plants, ferric chelate reductase, root cell wall, chlorosis, iron nutrition
MeSH terms
Cell Wall, Chlorophyll, Iron, Phenols, Plant Proteins, Plant Roots, Plant Shoots, Trifolium, FMN Reductase

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review