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Cited by 18Plant nutrient uptake and metabolismPhotosynthetic Processes and MechanismsNitrogen and Sulfur Effects on Brassica

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Experimental Evidence of Phospho enol pyruvate Resynthesis from Pyruvate in Illuminated Leaves

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Tcherkez, Guillaume, Mahé, Aline, Boex-Fontvieille, Edouard, Gout, Elisabeth, Guérard, Florence, Bligny, Richard·2011·Plant Physiology
DOI 10.1104/pp.111.180711PMC PMC3165900
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Abstract

Day respiration is the cornerstone of nitrogen assimilation since it provides carbon skeletons to primary metabolism for glutamate (Glu) and glutamine synthesis. However, recent studies have suggested that the tricarboxylic acid pathway is rate limiting and mitochondrial pyruvate dehydrogenation is partly inhibited in the light. Pyruvate may serve as a carbon source for amino acid (e.g. alanine) or fatty acid synthesis, but pyruvate metabolism is not well documented, and neither is the possible resynthesis of phosphoenolpyruvate (PEP). Here, we examined the capacity of pyruvate to convert back to PEP using 13C and 2H labeling in illuminated cocklebur (Xanthium strumarium) leaves. We show that the intramolecular labeling pattern in Glu, 2-oxoglutarate, and malate after 13C-3-pyruvate feeding was consistent with 13C redistribution from PEP via the PEP-carboxylase reaction. Furthermore, the deuterium loss in Glu after 2H3-13C-3-pyruvate feeding suggests that conversion to PEP and back to pyruvate washed out 2H atoms to the solvent. Our results demonstrate that in cocklebur leaves, PEP resynthesis occurred as a flux from pyruvate, approximately 0.5‰ of the net CO2 assimilation rate. This is likely to involve pyruvate inorganic phosphate dikinase and the fundamental importance of this flux for PEP and inorganic phosphate homeostasis is discussed.

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Metadata

Authors
Tcherkez, G., Mahé, A., Boex-Fontvieille, E., Gout, E., Guérard, F., & Bligny, R.
Affiliations
  • Université Paris-Sud (FR)
  • Institut de Biologie des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8618 (G.T., A.M., E.B.-F.), and Plateforme Métabolisme-Métabolome, Institut Fédératif de Recherche 87 (G.T., F.G.), Université Paris-Sud, 91405 Orsay cedex, France; Institut Universitaire de France, 75005 Paris, France (G.T.); Laboratoire de Physiologie Cellulaire Végétale, Unité Mixte de Reche
ORCID
Venue
Plant Physiology
Publisher
Oxford University Press (OUP)
Published
2011-07-05
Volume
157
Issue
1
Pages
86-95
ISSN
  • 0032-0889 (ISSN-L)
  • 1532-2548
DOI
10.1104/pp.111.180711
PMC
PMC3165900
OpenAlex
W2077728914
Cited by
18
References
0
Citations by year
2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 3
FWCI
1.93
Type
journal article
Language
en
Open access
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • PLANT PHYSIOLOGY (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Nitrogen and Sulfur Effects on Brassica
Keywords
pyruvate, phosphoenolpyruvate carboxylase, nitrogen assimilation, glutamate synthesis, deuterium labeling, cocklebur, Xanthium strumarium, inorganic phosphate homeostasis, TCA cycle, alpha-ketoglutarate, malate
MeSH terms
Carbon Isotopes, Phosphoenolpyruvate, Plant Leaves, Pyruvic Acid

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CoChat. (2026). Citation check: Experimental Evidence of Phospho enol pyruvate Resynthesis from Pyruvate in Illuminated Leaves. https://app.cochat.ai/papers/10.1104/pp.111.180711
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Tcherkez, G., Mahé, A., Boex-Fontvieille, E., Gout, E., Guérard, F., & Bligny, R. (2011). Experimental Evidence of Phospho enol pyruvate Resynthesis from Pyruvate in Illuminated Leaves. Plant Physiology. https://doi.org/10.1104/pp.111.180711