The response of diatom central carbon metabolism to nitrogen starvation is different from that of green algae and higher plants
Open accessAbstract
The availability of nitrogen varies greatly in the ocean and limits primary productivity over large areas. Diatoms, a group of phytoplankton that are responsible for about 20% of global carbon fixation, respond rapidly to influxes of nitrate and are highly successful in upwelling regions. Although recent diatom genome projects have highlighted clues to the success of this group, very little is known about their adaptive response to changing environmental conditions. Here, we compare the proteome of the marine diatom Thalassiosira pseudonana (CCMP 1335) at the onset of nitrogen starvation with that of nitrogen-replete cells using two-dimensional gel electrophoresis. In total, 3,310 protein spots were distinguishable, and we identified 42 proteins increasing and 23 decreasing in abundance (greater than 1.5-fold change; P < 0.005). Proteins involved in the metabolism of nitrogen, amino acids, proteins, and carbohydrates, photosynthesis, and chlorophyll biosynthesis were represented. Comparison of our proteomics data with the transcriptome response of this species under similar growth conditions showed good correlation and provided insight into different levels of response. The T. pseudonana response to nitrogen starvation was also compared with that of the higher plant Arabidopsis (Arabidopsis thaliana), the green alga Chlamydomonas reinhardtii, and the cyanobacterium Prochlorococcus marinus. We have found that the response of diatom carbon metabolism to nitrogen starvation is different from that of other photosynthetic eukaryotes and bears closer resemblance to the response of cyanobacteria.
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In the marine diatom T. pseudonana, proteins for nitrate and nitrite reduction were decreased during nitrogen starvation, while Glu synthase and Gln synthetase were increased.
“In the marine diatom T. pseudonana, proteins for nitrate and nitrite reduction were decreased during nitrogen starvation, while Glu synthase and Gln synthetase were increased (Hockin et al., 2012).”
From Responses of<i>Nannochloropsis oceanica</i>IMET1 to Long-Term Nitrogen Starvation and Recovery (2013) · cites this paper as Hockin et al., 2012
N. oceanica IMET1 was able to employ multiple approaches to scavenge various forms of inorganic nitrogen in the culture medium, similar to the response of the marine diatom T. pseudonana to nitrogen starvation.
“Thus, N. oceanica IMET1 was able to employ multiple approaches to scavenge various forms of inorganic nitrogen in the culture medium, similar to the response of the marine diatom T. pseudonana to nitrogen starvation (Hockin et al., 2012).”
From Responses of<i>Nannochloropsis oceanica</i>IMET1 to Long-Term Nitrogen Starvation and Recovery (2013) · cites this paper as Hockin et al., 2012
Different responses of the TCA cycle in C. reinhardtii and T. pseudonana to nitrogen starvation have been observed, showing that the flux of the TCA cycle may be adaptable.
“Different responses of the TCA cycle in C. reinhardtii and T. pseudonana to nitrogen starvation have been observed (Miller et al., 2010;Hockin et al., 2012), showing that the flux of the TCA cycle may be adaptable.”
From Responses of<i>Nannochloropsis oceanica</i>IMET1 to Long-Term Nitrogen Starvation and Recovery (2013) · cites this paper as Hockin et al., 2012
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References 60 · Cited by 372
Research works printed but not linked: 2
- Harrison PJ,Berges JA (2005) Marine culture medium. In RA Andersen, eds, Algal Culturing Techniques. Academic Press, San Diego, pp 21-33.
- Zadykowicz E,Robertson D (2005). Phylogenetic relationships amongst glutamate synthase enzymes. 44th Northeast Algal Symposium, Rockland, Maine, pp 41.
- Authors
- Hockin, N. L., Mock, T., Mulholland, F., Kopriva, S., & Malin, G.
- Affiliations
- John Innes Centre (GB)
- Laboratory for Global Marine and Atmospheric Chemistry, School of Environmental Sciences (N.L.H., G.M.), and School of Environmental Sciences (T.M.), University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, United Kingdom; John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom (N.L.H., S.K.); Institute of Food Research, Norwich Research Park, Colney, Norwich N
- ORCID
- Malin, Gill
- Venue
- PLANT PHYSIOLOGY
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-11-07
- Volume
- 158
- Issue
- 1
- Pages
- 299-312
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- PMC
- PMC3252072
- OpenAlex
- W2124390857
- Cited by
- 372
- References
- 60
- Citations by year
- 2026: 13 · 2025: 16 · 2024: 27 · 2023: 24 · 2022: 24 · 2021: 18 · 2020: 34 · 2019: 35 · 2018: 27 · 2017: 32 · 2016: 33 · 2015: 28 · 2014: 28 · 2013: 25 · 2012: 8
- FWCI
- 8.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- PLANT PHYSIOLOGY (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UEA Digital Repository (University of East Anglia) (submittedVersion)
- Funders
- University of East Anglia
- Directorate for Biological Sciences
- Biotechnology and Biological Sciences Research Council (BBS/E/F/00042291)
- Natural Environment Research Council (NE/B501039/1)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Diatoms and Algae Research
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal ecosystems
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- Thalassiosira pseudonana, nitrogen starvation, central carbon metabolism, comparative proteomics, Arabidopsis thaliana, Chlamydomonas reinhardtii, Prochlorococcus marinus, cyanobacteria, photosynthesis, chlorophyll biosynthesis, amino acid metabolism, carbohydrate metabolism, transcriptome comparison, phytoplankton adaptation
- MeSH terms
- Amino Acids, Carbon, Nitrogen, Photosynthesis, Proteins, Electrophoresis, Gel, Two-Dimensional, Chlamydomonas reinhardtii, Arabidopsis, Diatoms, Proteome, Aquatic Organisms
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