Cytoplasmic Free Ca2+ in Arabidopsis Roots Changes in Response to Touch but Not Gravity

Abstract

Changes in cytoplasmic Ca2+ concentration ([Ca2+]i) have been proposed to be involved in signal transduction pathways in response to a number of stimuli, including gravity and touch. The current hypothesis proposes that the development of gravitropic bending is correlated with a redistribution of [Ca2+]i in gravistimulated roots. However, no study has demonstrated clearly the development of an asymmetry of this ion during root curvature. We tested this hypothesis by quantifying the temporal and spatial changes in [Ca2+]i in roots of living Arabidopsis seedlings using ultraviolet-confocal Ca2+-ratio imaging and vertical stage fluorescence microscopy to visualize root [Ca2+]i. We observed no changes in [Ca2+]i associated with the graviresponse whether monitored at the whole organ level or in individual cells in different regions of the root for up to 12 h after gravistimulation. However, touch stimulation led to transient increases in [Ca2+]i in all cell types monitored. The increases induced in the cap cells were larger and longer-lived than in cells in the meristematic or elongation zone. One millimolar La3+ and 100 [mu]M verapamil did not prevent these responses, whereas 5 mM EGTA or 50 [mu]M ruthenium red inhibited the transients, indicating an intracellular origin of the Ca2+ increase. These results suggest that, although touch responses of roots may be mediated through a Ca2+-dependent pathway, the gravitropic response is not associated with detectable changes in [Ca2+]i.