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Flavonoids Act as Negative Regulators of Auxin Transport in Vivo in Arabidopsis

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Brown, Dana E., Rashotte, Aaron M., Murphy, Angus S., Normanly, Jennifer, Tague, Brian W., Peer, Wendy A., Taiz, Lincoln, Muday, Gloria K.·2001·Plant Physiology
DOI 10.1104/pp.126.2.524
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Abstract

Polar transport of the plant hormone auxin controls many aspects of plant growth and development. A number of synthetic compounds have been shown to block the process of auxin transport by inhibition of the auxin efflux carrier complex. These synthetic auxin transport inhibitors may act by mimicking endogenous molecules. Flavonoids, a class of secondary plant metabolic compounds, have been suggested to be auxin transport inhibitors based on their in vitro activity. The hypothesis that flavonoids regulate auxin transport in vivo was tested in Arabidopsis by comparing wild-type (WT) and transparent testa (tt4) plants with a mutation in the gene encoding the first enzyme in flavonoid biosynthesis, chalcone synthase. In a comparison between tt4 and WT plants, phenotypic differences were observed, including three times as many secondary inflorescence stems, reduced plant height, decreased stem diameter, and increased secondary root development. Growth of WT Arabidopsis plants on naringenin, a biosynthetic precursor to those flavonoids with auxin transport inhibitor activity in vitro, leads to a reduction in root growth and gravitropism, similar to the effects of synthetic auxin transport inhibitors. Analyses of auxin transport in the inflorescence and hypocotyl of independent tt4 alleles indicate that auxin transport is elevated in plants with a tt4mutation. In hypocotyls of tt4, this elevated transport is reversed when flavonoids are synthesized by growth of plants on the flavonoid precursor, naringenin. These results are consistent with a role for flavonoids as endogenous regulators of auxin transport.

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Research works printed but not linked: 51

  1. Bennett, 1998, Going the distance with auxin: unravelling the molecular basis of auxin transport., Philos Trans R Soc Lond B, 353, 1511, 10.1098/rstb.1998.0306 · DOI
  2. Bernasconi, 1996, Effect of synthetic and natural protein tyrosine kinase inhibitors on auxin efflux in zucchini (Cucurbita pepo) hypocotyls., Physiol Plant, 96, 205, 10.1111/j.1399-3054.1996.tb00203.x · DOI
  3. Brown, 1998, Testing the hypothesis that flavonoids regulate polar auxin transport in vivo using Arabidopsis thaliana. MS thesis.
  4. Burbulis, 1996, A null mutation in the first enzyme of flavonoid biosynthesis does not affect male fertility in Arabidopsis., Plant Cell, 8, 1013
  5. Chen, 1988, A rapid and simple procedure for purification of indole-3-acetic acid prior to GC-SIM-MS analysis., Plant Physiol, 86, 822, 10.1104/pp.86.3.822 · DOI · Paper page
  6. Chen, 1998, The Arabidopsis thaliana AGRAVITROPIC 1 gene encodes a component of the polar-auxin-transport efflux carrier., Proc Natl Acad Sci USA, 95, 15112, 10.1073/pnas.95.25.15112 · DOI · Paper page
  7. Chory, 1990, Mutations in the DET1 gene affect cell-type-specific expression of light-regulated genes and chloroplast development in Arabidopsis., Proc Natl Acad Sci USA, 87, 8776, 10.1073/pnas.87.22.8776 · DOI
  8. Cohen, 1986, 13C6-[Benzene ring]-indole-3-acetic acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic acid in plants., Plant Physiol, 80, 14, 10.1104/pp.80.1.14 · DOI · Paper page
  9. Deruère, 1999, The RCN1-encoded A subunit of protein phosphatase 2A increases phosphatase activity in vivo., Plant J, 20, 389, 10.1046/j.1365-313x.1999.00607.x · DOI
  10. Dixon, 1996, Cytoplasmic orientation of the naphthylphthalamic acid-binding protein in zucchini plasma membrane vesicles., Plant Physiol, 112, 421, 10.1104/pp.112.1.421 · doi:10.1104/pp.112.1.421
  11. Feinbaum, 1988, Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene., Mol Cell Biol, 8, 1985
  12. Feinbaum, 1991, High intensity and blue light regulated expression of chimeric chalcone synthase genes in transgenic Arabidopsis thaliana plants., Mol Gen Genet, 226, 449, 10.1007/BF00260658 · doi:10.1007/bf00260658
  13. Fischer, 1997, Induction of zygotic polyembryos in wheat: influence of auxin polar transport., Plant Cell, 9, 1767, 10.2307/3870523 · doi:10.2307/3870523
  14. Furuya, 1962, Isolation from peas of co-factors and inhibitors of indolyl-3-acetic acid oxidase., Nature, 193, 456, 10.1038/193456a0 · doi:10.1038/193456a0
  15. Garbers, 1996, A mutation in protein phosphatase 2A regulatory subunit A affects auxin transport in Arabidopsis., EMBO J, 15, 2115, 10.1002/j.1460-2075.1996.tb00565.x · DOI · Paper page
  16. Hirsch, 1989, Early nodulin genes are inducible in alfalfa root outgrowths elicited by auxin transport inhibitors., Proc Natl Acad Sci USA, 86, 1244, 10.1073/pnas.86.4.1244 · DOI · Paper page
  17. Hirsch, 1994, Plant hormones and nodulation: what's the connection?, Plant Mol Biol, 26, 5, 10.1007/BF00039514 · DOI
  18. Jackson, 1995, Isolation of Arabidopsis mutants altered in the light-regulation of chalcone synthase gene expression using a transgenic screening approach., Plant J, 8, 369, 10.1046/j.1365-313X.1995.08030369.x · doi:10.1046/j.1365-313x.1995.08030369.x
  19. Jacobs, 1988, Naturally occurring auxin transport regulators., Science, 241, 346, 10.1126/science.241.4863.346 · DOI · Paper page
  20. Katekar, 1976, Inhibitors of the geotropic response in plants: a correlation of molecular structures., Phytochemistry, 15, 1421, 10.1016/S0031-9422(00)88906-2 · doi:10.1016/s0031-9422(00)88906-2
  21. Kubasek, 1992, Regulation of flavonoid biosynthetic genes in germinating Arabidopsis seedlings., Plant Cell, 4, 1229, 10.2307/3869409 · doi:10.2307/3869409
  22. Lomax, 1995, Auxin transport., 509, 10.1007/978-94-011-0473-9_24 · DOI · Paper page
  23. Marchant, 1999, AUX1 regulates root gravitropism in Arabidopsis by facilitating auxin uptake within root apical tissues., EMBO J, 18, 2066, 10.1093/emboj/18.8.2066 · DOI · Paper page
  24. Marigo, 1977, Relations polyphenols-croissance: mise en evidence d'un effet inhibiteur des composes phenoliques sur le transport polarise de l'auxine., Physiol Plant, 41, 197, 10.1111/j.1399-3054.1977.tb05557.x · doi:10.1111/j.1399-3054.1977.tb05557.x
  25. Mathesius, 1998, Auxin transport inhibition precedes root nodule formation in white clover roots and is regulated by flavonoids and derivatives of chitin oligosaccharides., Plant J, 14, 23, 10.1046/j.1365-313X.1998.00090.x · DOI · Paper page
  26. Mitchell, 1975, Evidence for three different systems of movement of indoleacetic acid in intact roots of Phaseolus coccineus., Physiol Plant, 33, 290, 10.1111/j.1399-3054.1975.tb03171.x · doi:10.1111/j.1399-3054.1975.tb03171.x
  27. Muday, 2000, Interactions between the actin cytoskeleton and an auxin transport protein., 541, 10.1007/978-94-015-9460-8_30 · DOI
  28. Mumford, 1961, An inhibitor of indoleacetic acid oxidase from pea tips., Plant Physiol, 36, 752, 10.1104/pp.36.6.752 · DOI
  29. Murphy, 2000, Regulation of auxin transport by aminopeptidases and endogenous flavonoids., Planta, 211, 315, 10.1007/s004250000300 · DOI · Paper page
  30. Murphy, 1995, A new vertical mesh transfer technique for metal tolerance studies in Arabidopsis: ecotypic variation and copper-sensitive mutants., Plant Physiol, 108, 29, 10.1104/pp.108.1.29 · DOI
  31. Normanly, 1995, Rethinking auxin biosynthesis and metabolism., Plant Physiol, 107, 1, 10.1104/pp.107.2.323 · doi:10.1104/pp.107.2.323
  32. Okada, 1991, Requirement of the auxin polar transport system in early stages of Arabidopsis floral bud formation., Plant Cell, 3, 677, 10.2307/3869249 · doi:10.2307/3869249 · Paper page
  33. Palme, 1999, PIN-pointing the molecular basis of auxin transport., Curr Opin Plant Biol, 2, 375, 10.1016/S1369-5266(99)00008-4 · DOI · Paper page
  34. Peer, 2001, Flavonoid accumulation patterns of transparent testa mutants of Arabidopsis., Plant Physiol, 126, 536, 10.1104/pp.126.2.536 · DOI · Paper page
  35. Rashotte, 2000, Basipetal auxin transport is required for gravitropism in roots of Arabidopsis., Plant Physiol, 122, 481, 10.1104/pp.122.2.481 · DOI · Paper page
  36. Rayle, 1969, Effect of auxins on the auxin transport system in coleoptiles., Planta, 87, 49, 10.1007/BF00386963 · DOI
  37. Reed, 1998, Inhibition of auxin movement from the shoot into the root inhibits lateral root development in Arabidopsis., Plant Physiol, 118, 1369, 10.1104/pp.118.4.1369 · DOI · Paper page
  38. Rubery, 1990, Auxin transport and its regulation by flavonoids., 428, 10.1007/978-3-642-74545-4_50 · doi:10.1007/978-3-642-74545-4_50
  39. Rubery, 1990, Phytotropins: receptors and endogenous ligands., Symp Soc Exp Biol, 44, 119
  40. Ruegger, 1997, Reduced naphthylphthalamic acid binding in the tir3 mutant of Arabidopsis is associated with a reduction in polar auxin transport and diverse morphological defects., Plant Cell, 9, 745
  41. Sakuta, 2000, Transcriptional control of chalcone synthase by environmental stimuli., J Plant Res, 113, 327, 10.1007/PL00013943 · doi:10.1007/pl00013943
  42. Saslowsky, 2000, An allelic series for the chalcone synthase locus in Arabidopsis., Gene, 255, 127, 10.1016/S0378-1119(00)00304-8 · doi:10.1016/s0378-1119(00)00304-8
  43. Schmid, 1990, Developmental and environmental regulation of a bean chalcone synthase promoter in transgenic tobacco., Plant Cell, 2, 619
  44. Shirley, 1996, Flavonoid biosynthesis: “new” functions for an “old” pathway., Trends Plant Sci, 1, 377
  45. Shirley, 1995, Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis., Plant J, 8, 659, 10.1046/j.1365-313X.1995.08050659.x · DOI · Paper page
  46. Stafford, 1990, Flavonoid Metabolism.
  47. Stenlid, 1963, The effects of flavonoid compounds on oxidative phosphorylation and on the enzymatic destruction of indole-acetic acid., Physiol Plant, 16, 110, 10.1111/j.1399-3054.1963.tb08295.x · DOI
  48. Stenlid, 1976, Effects of flavonoids on the polar transport of auxins., Physiol Plant, 38, 262, 10.1111/j.1399-3054.1976.tb04001.x · DOI
  49. Tsurumi, 1978, Transport of 14C-labeled indoleacetic acid in Vicia root segments., Plant Cell Physiol, 19, 1195
  50. von Arnim, 1996, Light control of seedling development., Annu Rev Plant Physiol Plant Mol Biol, 47, 215, 10.1146/annurev.arplant.47.1.215 · DOI · Paper page
  51. Yang, 1992, In-situ localization of chalcone synthase mRNA in pea root nodule development., Plant J, 2, 143, 10.1111/j.1365-313X.1992.00143.x · doi:10.1111/j.1365-313x.1992.00143.x

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Metadata

Authors
Brown, D. E., Rashotte, A. M., Murphy, A. S., Normanly, J., Tague, B. W., Peer, W. A., Taiz, L., & Muday, G. K.
Venue
Plant Physiology
Publisher
Oxford University Press (OUP)
Published
2001-06-01
Volume
126
Issue
2
Pages
524-535
ISSN
  • 0032-0889 (ISSN-L)
  • 1532-2548
DOI
10.1104/pp.126.2.524
OpenAlex
W2068011088
Cited by
799
References
1
FWCI
25.16
Type
journal article
Language
en
Open access
bronze
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)

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CoChat. (2026). Citation check: Flavonoids Act as Negative Regulators of Auxin Transport in Vivo in Arabidopsis. https://app.cochat.ai/papers/10.1104/pp.126.2.524
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Brown, D. E., Rashotte, A. M., Murphy, A. S., Normanly, J., Tague, B. W., Peer, W. A., Taiz, L., & Muday, G. K. (2001). Flavonoids Act as Negative Regulators of Auxin Transport in Vivo in Arabidopsis. Plant Physiology. https://doi.org/10.1104/pp.126.2.524

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