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Refinement of the Triphenyl Tetrazolium Chloride Method of Determining Cold Injury
Open accessAbstract
The method of evaluating cold injury in woody plants by the use of triphenyl tetrazolium chloride has been refined to eliminate bias associated with visual differentiation between varying degrees of tetrazolium reduction and to predict tissue survival at a later date. An advantage of the method described here is that a small amount of tissue (50-100 mg) is required; this, therefore, allows for hardiness determinations at precise locations on the plant. The high correlation between cold injury and triphenyl tetrazolium chloride reduction may be due to cofactor and substrate limitations rather than inactivation of dehydrogenases.
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Citations
Cited by
Assessing root death and root system dynamics in a study of grape canopy pruning
COMAS, L. H., EISSENSTAT, D. M., & LAKSO, A. N. (2000) · New Phytologist · doi:10.1046/j.1469-8137.2000.00679.x · Cited by 335
Gong, M., Chen, S.-N., Song, Y.-Q., & Li, Z.-G. (1997) · Australian Journal of Plant Physiology · doi:10.1071/pp96118 · Cited by 159
Metadata
- Authors
- Steponkus, P. L., & Lanphear, F. O.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1967-10-01
- Volume
- 42
- Issue
- 10
- Pages
- 1423-1426
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2011045841
- Cited by
- 490
- References
- 0
- FWCI
- 4.51
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Refinement of the Triphenyl Tetrazolium Chloride Method of Determining Cold Injury. https://app.cochat.ai/papers/10.1104/pp.42.10.1423
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- Steponkus, P. L., & Lanphear, F. O. (1967). Refinement of the Triphenyl Tetrazolium Chloride Method of Determining Cold Injury. Plant Physiology. https://doi.org/10.1104/pp.42.10.1423
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.