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Refinement of the Triphenyl Tetrazolium Chloride Method of Determining Cold Injury

Open access
Steponkus, Peter L., Lanphear, F. O.·1967·Plant Physiology

Abstract

The method of evaluating cold injury in woody plants by the use of triphenyl tetrazolium chloride has been refined to eliminate bias associated with visual differentiation between varying degrees of tetrazolium reduction and to predict tissue survival at a later date. An advantage of the method described here is that a small amount of tissue (50-100 mg) is required; this, therefore, allows for hardiness determinations at precise locations on the plant. The high correlation between cold injury and triphenyl tetrazolium chloride reduction may be due to cofactor and substrate limitations rather than inactivation of dehydrogenases.

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Metadata

Authors
Steponkus, P. L., & Lanphear, F. O.
Venue
Plant Physiology
Published
1967-10-01
Volume
42
Issue
10
Pages
1423-1426
ISSN
  • 0032-0889 (ISSN-L)
  • 1532-2548
OpenAlex
W2011045841
Cited by
490
References
0
FWCI
4.51
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Refinement of the Triphenyl Tetrazolium Chloride Method of Determining Cold Injury. https://app.cochat.ai/papers/10.1104/pp.42.10.1423
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Steponkus, P. L., & Lanphear, F. O. (1967). Refinement of the Triphenyl Tetrazolium Chloride Method of Determining Cold Injury. Plant Physiology. https://doi.org/10.1104/pp.42.10.1423

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.