The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Enzymic Assay of 10−7 to 10−14 Moles of Sucrose in Plant Tissues
Open accessAbstract
Procedures are described for measuring sucrose in plant extracts or freeze-dried tissue in the range between 10(-7) and 10(-14) moles. The method is based on the destruction of pre-existing glucose and fructose, followed by the hydrolysis of sucrose and reduction of NADP(+) by a series of coupled enzymic reactions. Depending on the sensitivity required, the NADPH is determined directly with a spectrophotometer or a fluorometer, or is amplified as much as 30,000 times before fluorometric assay. The procedures suggested for the macro level are simpler than current methods, and those suggested for microanalysis are several orders of magnitude more sensitive.With this technique, single palisade parenchyma cells and single spongy parenchyma cells of Vicia faba leaflets were each found to contain about 2.2 pmoles of sucrose.
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Citations
Cites
A Flexible System of Enzymatic Analysis
(1972) · Elsevier eBooks · doi:10.1016/b978-0-12-457950-7.x5001-3 · Cited by 4,051
Direct microdetermination of sucrose
van Handel, E. (1968) · Analytical Biochemistry · doi:10.1016/0003-2697(68)90317-5 · Cited by 729
Storage of sugars in stalks of sugar cane
Glasziou, K. T., & Gayler, K. R. (1972) · The Botanical Review · doi:10.1007/bf02859248 · Cited by 191
Finch, P., Yuen, R., Schachter, H., & Moscarello, M. A. (1969) · Analytical Biochemistry · doi:10.1016/0003-2697(69)90269-3 · Cited by 149
Stabilizing the alkali-generated fluorescent derivatives of NAD and NADP
Lowry, O. H., & Carter, J. G. (1974) · Analytical Biochemistry · doi:10.1016/0003-2697(74)90319-4 · Cited by 30
Self-citationCompartmentation in Vicia faba Leaves. III. Photosynthesis in the Spongy and Palisade Parenchyma
Outlaw, W., & Fisher, D. (1975) · Australian Journal of Plant Physiology · doi:10.1071/pp9750435 · Cited by 14
Self-citation
Cited by
Yendrek, C. R., Tomaz, T., Montes, C. M., Cao, Y., Morse, A. M., Brown, P. J., McIntyre, L. M., Leakey, A. D. B., & Ainsworth, E. A. (2017) · Plant Physiology · doi:10.1104/pp.16.01447 · Cited by 308
Current Understandings on Magnesium Deficiency and Future Outlooks for Sustainable Agriculture
Chaudhry, A. H., Nayab, S., Hussain, S. B., Ali, M., & Pan, Z. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22041819 · Cited by 100
AMTHOR, J. S., MITCHELL, R. J., RUNION, G. B., ROGERS, H. H., PRIOR, S. A., & WOOD, C. W. (1994) · New Phytologist · doi:10.1111/j.1469-8137.1994.tb02990.x · Cited by 60
Metadata
- Authors
- Jones, M. G. K., Outlaw, W. H., & Lowry, O. H.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1977-09-01
- Volume
- 60
- Issue
- 3
- Pages
- 379-383
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2044400584
- Cited by
- 485
- References
- 6
- FWCI
- 8.21
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Jones, M. G. K., Outlaw, W. H., & Lowry, O. H. (1977). Enzymic Assay of 10−7 to 10−14 Moles of Sucrose in Plant Tissues. Plant Physiology. https://doi.org/10.1104/pp.60.3.379
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