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Formulae for Determination of Chlorophyllous Pigments Extracted with N,N-Dimethylformamide
Open accessAbstract
The extraction of chlorophylls in higher plant tissue using N,N-dimethylformamide expedites the process and enables the determination of small samples with low pigment level.Absorption spectra of Chl a, Chl b, and Pchl and of their acidified derivatives, the phaeophytins, were recorded. Conversion of Chl b to its corresponding acidified product occurs much more slowly than that of Chl a and Pchl. When acidified, Pchl differs from Chl a and Chl b by the disappearance of the red band in the absorption spectrum. Specific extinction coefficients were determined and formulae for quantitative determination of pigments concentrations were developed. When concentrations of pigments are low, as in etiolated plant material, the absorption spectra of the chlorophylls can be distorted due to the presence of other substances simultaneously extracted; formulae for pigment determination under such circumstances were also derived.
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Cited by
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Li, Q., & Kubota, C. (2009) · Environmental and Experimental Botany · doi:10.1016/j.envexpbot.2009.06.011 · Cited by 914
Green-light supplementation for enhanced lettuce growth under red- and blue-light-emitting diodes
Kim, H.-H., Goins, G. D., Wheeler, R. M., & Sager, J. C. (2004) · HortScience · doi:10.21273/hortsci.39.7.1617 · Cited by 527
Larue, C., Castillo-Michel, H., Sobanska, S., Cécillon, L., Bureau, S., Barthès, V., Ouerdane, L., Carrière, M., & Sarret, G. (2014) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2013.10.053 · Cited by 441
Rius, S. P., Casati, P., Iglesias, A. A., & Gomez-Casati, D. F. (2006) · Plant Molecular Biology · doi:10.1007/s11103-006-0060-5 · Cited by 94
Tsuzuki, T., Takahashi, K., Inoue, S.-I., Okigaki, Y., Tomiyama, M., Hossain, M. A., Shimazaki, K.-I., Murata, Y., & Kinoshita, T. (2011) · Journal of Plant Research · doi:10.1007/s10265-011-0426-x · Cited by 83
“The chlorophyll content of rosette leaves from 4-week-old plants was determined as previously described (Moran 1982).”
Metadata
- Authors
- Moran, R.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1982-06-01
- Volume
- 69
- Issue
- 6
- Pages
- 1376-1381
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2067075632
- Cited by
- 1,230
- References
- 0
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Moran, R. (1982). Formulae for Determination of Chlorophyllous Pigments Extracted with N,N-Dimethylformamide. Plant Physiology. https://doi.org/10.1104/pp.69.6.1376
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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