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Subunit Structure of Spinach Leaf ADPglucose Pyrophosphorylase
Open accessAbstract
To initiate structural studies of the ADPglucose pyrophosphorylase from spinach an improved purification procedure was devised. The modified purification scheme allowed the isolation of 20 to 30 milligrams pure enzyme from 10 kilogram of spinach leaves. Electrophoresis of the purified enzyme confirmed an earlier study which showed that the enzyme was putatively composed of two subunits (Copeland L, J Preiss 1981 Plant Physiol 68: 996-1001). The two subunits migrate as 51 and 54 kilodalton proteins upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both proteins can be detected on Western blots of leaf homogenates prepared under denaturing conditions suggesting that both subunits exist in vivo. Anion-exchange chromatography in the presence of urea allowed resolution of the 51 and 54 kilodalton proteins. They possess different N-terminal amino acid sequences and tryptic peptide maps. Western blot analysis reveals that the 51 and 54 kilodalton proteins are antigenically dissimilar. The 51 but not the 54 kilodalton protein is immunologically related to the ADPglucose pyrophosphorylase from maize endosperm and potato tuber.
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Citations
Cited by
Hendriks, J. H. M., Kolbe, A., Gibon, Y., Stitt, M., & Geigenberger, P. (2003) · Plant Physiology · doi:10.1104/pp.103.024513 · Cited by 458
Kolbe, A., Tiessen, A., Schluepmann, H., Paul, M., Ulrich, S., & Geigenberger, P. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0503410102 · Cited by 408
Lin, T.-P., Caspar, T., Somerville, C., & Preiss, J. (1988) · Plant Physiology · doi:10.1104/pp.86.4.1131 · Cited by 338
Self-citationADP-Glucose Pyrophosphorylase: A Regulatory Enzyme for Plant Starch Synthesis
Ballicora, M. A., Iglesias, A. A., & Preiss, J. (2004) · Photosynthesis Research · doi:10.1023/b:pres.0000011916.67519.58 · Cited by 324
Self-citationSucrose signaling in higher plants
Yoon, J., Cho, L.-H., Tun, W., Jeon, J.-S., & An, G. (2021) · Plant Science · doi:10.1016/j.plantsci.2020.110703 · Cited by 323
Metadata
- Authors
- Morell, M. K., Bloom, M., Knowles, V., & Preiss, J.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1987-09-01
- Volume
- 85
- Issue
- 1
- Pages
- 182-187
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2014890562
- Cited by
- 164
- References
- 0
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- bronze
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