Influence of Photorespiration on ATP/ADP Ratios in the Chloroplasts, Mitochondria, and Cytosol, Studied by Rapid Fractionation of Barley (Hordeum vulgare) Protoplasts
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Using the principle described by R McC Lilley, M Stitt, G Mader, HW Heldt (1982 Plant Physiol 70: 965-970), an apparatus for rapid fractionation of barley leaf (Hordeum vulgare) protoplasts by membrane filtration was built. From studies of ATP/ADP ratios, it is concluded that the quenching of metabolic reactions is very fast, making it possible to use the method for studies on metabolic interactions between different compartments in plant cells. The fractionation method was used to study the influence of photorespiration on ATP/ADP ratios in the chloroplasts, mitochondria, and cytosol of barley leaf protoplasts. The cytosolic ATP/ADP ratio was higher under photorespiratory conditions than under nonphotorespiratory conditions. Aminoacetonitrile, an inhibitor of the photorespiratory conversion of glycine to serine, had a very small effect on the ATP/ADP ratios in the different subcellular compartments during photosynthesis in nonphotorespiratory conditions (saturating CO(2)). In photorespiratory conditions (limiting CO(2)), on the other hand, aminoacetonitrile increased the ATP/ADP ratio in the chloroplasts and decreased the ATP/ADP ratios in the mitochondria and the cytosol. These results are consistent with the hypothesis, that during photorespiration glycine oxidation is coupled to oxidative phosphorylation to provide ATP to the cytosol.
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Addition of oligomycin to barley protoplasts decreased the ATP-ADP ratio in both mitochondria and cytosol, whereas the chloroplast ATP-ADP ratio was unchanged.
“the addition of oligomycin to barley (Hordeum vulgare) protoplasts decreased the ATP-ADP ratio in both mitochondria and cytosol, whereas the chloroplast ATP-ADP ratio was unchanged (Gardeström and Wigge, 1988; Krömer and Heldt, 1991; Wigge et al., 1993).”
From Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light (2019) · cites this paper as Gardeström and Wigge, 1988
The Results passage on inhibitor treatment reports that AAN decreased mitochondrial and cytosolic ATP/ADP ratios under photorespiratory conditions, while slightly increasing the chloroplast ratio; it does not report an oligomycin treatment. Searches for “oligomycin” and for the claimed compartment-specific effect found no passage addressing oligomycin in our copy. (LLM, conf=0.76)
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References 27 · Cited by 186
Research works printed but not linked: 3
- GARDESTROM P, GE EDWARDS 1985 Leaf mitochondria (C3+C4+CAM). In R Douce, DA Day, eds, Encyclopedia of Plant Physiology, New Series, Vol 18, Higher Plant Cell Respiration. Springer-Verlag, Berlin, pp 314-346
- OLIVER DJ 1984 Evidence for the involvement of the mitochondrial electron transport chain in photorespiratory glycine oxidation. In C Sybesma, ed, Advances in Photosynthesis Research, Vol 3. W Junk, The Hague, pp 855- 858
- SICHER RC 1984 Glycolaldehyde inhibition of photosynthetic carbon assimi- lation by isolated chloroplasts and protoplasts. In C Sybesma, ed, Advances in Photosynthesis Research, Vol 3. W Junk, The Hague, pp 413-416
- Authors
- Gardeström, P., & Wigge, B.
- Affiliations
- Umeå University (SE)
- ORCID
- Gardeström, Per
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1988-09-01
- Volume
- 88
- Issue
- 1
- Pages
- 69-76
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- PMC
- PMC1055527
- OpenAlex
- W2054283978
- Cited by
- 186
- References
- 27
- Citations by year
- 2025: 3 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 7 · 2019: 5 · 2018: 5 · 2017: 10 · 2016: 5 · 2015: 1 · 2014: 3 · 2013: 4 · 2012: 3
- FWCI
- 1.72
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- PLANT PHYSIOLOGY (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Keywords
- photorespiration, ATP/ADP ratio, barley protoplasts, chloroplasts, mitochondria, cytosol, subcellular compartmentalization, aminoacetonitrile, glycine oxidation, oxidative phosphorylation, photosynthesis, CO2 limitation
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