The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Membrane Protein Transport between the Endoplasmic Reticulum and the Golgi in Tobacco Leaves Is Energy Dependent but Cytoskeleton Independent: Evidence from Selective Photobleaching
Open accessAbstract
The mechanisms that control protein transport between the endoplasmic reticulum (ER) and the Golgi apparatus are poorly characterized in plants. Here, we examine in tobacco leaves the structural relationship between Golgi and ER membranes using electron microscopy and demonstrate that Golgi membranes contain elements that are in close association and/or in direct contact with the ER. We further visualized protein trafficking between the ER and the Golgi using Golgi marker proteins tagged with green fluorescent protein. Using photobleaching techniques, we showed that Golgi membrane markers constitutively cycle to and from the Golgi in an energy-dependent and N-ethylmaleimide-sensitive manner. We found that membrane protein transport toward the Golgi occurs independently of the cytoskeleton and does not require the Golgi to be motile along the surface of the ER. Brefeldin A treatment blocked forward trafficking of Golgi proteins before their redistribution into the ER. Our results indicate that in plant cells, the Golgi apparatus is a dynamic membrane system whose components continuously traffic via membrane trafficking pathways regulated by brefeldin A- and N-ethylmaleimide-sensitive machinery.
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Cites
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Self-citationER-to-Golgi transport visualized in living cells
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Self-citationProtein Sorting by Transport Vesicles
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Self-citationCoat Proteins and Vesicle Budding
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Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network†
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Cited by
Nelson, B. K., Cai, X., & Nebenführ, A. (2007) · The Plant Journal · doi:10.1111/j.1365-313x.2007.03212.x · Cited by 2,083
BI-DIRECTIONAL PROTEIN TRANSPORT BETWEEN THE ER AND GOLGI
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Organization of the ER–Golgi interface for membrane traffic control
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Self-citationBrefeldin A: Deciphering an Enigmatic Inhibitor of Secretion
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Metadata
- Authors
- Brandizzi, F., Snapp, E. L., Roberts, A. G., Lippincott-Schwartz, J., & Hawes, C.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-06
- Volume
- 14
- Issue
- 6
- Pages
- 1293-1309
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2134859485
- Cited by
- 305
- References
- 45
- FWCI
- 5.94
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Brandizzi, F., Snapp, E. L., Roberts, A. G., Lippincott-Schwartz, J., & Hawes, C. (2002). Membrane Protein Transport between the Endoplasmic Reticulum and the Golgi in Tobacco Leaves Is Energy Dependent but Cytoskeleton Independent: Evidence from Selective Photobleaching. The Plant Cell. https://doi.org/10.1105/tpc.001586
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