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Cited by 520

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The Short-Chain Alcohol Dehydrogenase ABA2 Catalyzes the Conversion of Xanthoxin to Abscisic Aldehyde[W]

Open access
González-Guzmán, Miguel, Apostolova, Nadezda, Bellés, José M., Barrero, José M., Piqueras, Pedro, Ponce, María R., Micol, José L., Serrano, Ramón, Rodríguez, Pedro L.·2002·The Plant Cell

Abstract

Mutants able to germinate and perform early growth in medium containing a high NaCl concentration were identified during the course of two independent screenings and named salt resistant (sre) and salobreño (sañ). The sre and sañ mutants also were able to germinate in high-osmoticum medium, indicating that they are osmotolerant in a germination assay. Complementation analyses revealed that sre1-1, sre1-2, sañ3-1, and sañ3-2 were alleles of the abscisic acid (ABA) biosynthesis ABA2 gene. A map-based cloning strategy allowed the identification of the ABA2 gene and molecular characterization of four new aba2 alleles. The ABA2 gene product belongs to the family of short-chain dehydrogenases/reductases, which are known to be NAD- or NADP-dependent oxidoreductases. Recombinant ABA2 protein produced in Escherichia coli exhibits a K m value for xanthoxin of 19 μM and catalyzes in a NAD-dependent manner the conversion of xanthoxin to abscisic aldehyde, as determined by HPLC–mass spectrometry. The ABA2 mRNA is expressed constitutively in all plant organs examined and is not upregulated in response to osmotic stress. The results of this work are discussed in the context of previous genetic and biochemical evidence regarding ABA biosynthesis, confirming the xanthoxin→abscisic aldehyde→ABA transition as the last steps of the major ABA biosynthetic pathway.

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Metadata

Authors
González-Guzmán, M., Apostolova, N., Bellés, J. M., Barrero, J. M., Piqueras, P., Ponce, M. R., Micol, J. L., Serrano, R., & Rodríguez, P. L.
Venue
The Plant Cell
Published
2002-08-01
Volume
14
Issue
8
Pages
1833-1846
ISSN
  • 1040-4651 (ISSN-L)
  • 1532-298X
OpenAlex
W2163183563
Cited by
520
References
1
FWCI
4.21
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: The Short-Chain Alcohol Dehydrogenase ABA2 Catalyzes the Conversion of Xanthoxin to Abscisic Aldehyde[W]. https://app.cochat.ai/papers/10.1105/tpc.002477
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González-Guzmán, M., Apostolova, N., Bellés, J. M., Barrero, J. M., Piqueras, P., Ponce, M. R., Micol, J. L., Serrano, R., & Rodríguez, P. L. (2002). The Short-Chain Alcohol Dehydrogenase ABA2 Catalyzes the Conversion of Xanthoxin to Abscisic Aldehyde[W]. The Plant Cell. https://doi.org/10.1105/tpc.002477

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