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The Short-Chain Alcohol Dehydrogenase ABA2 Catalyzes the Conversion of Xanthoxin to Abscisic Aldehyde[W]
Open accessAbstract
Mutants able to germinate and perform early growth in medium containing a high NaCl concentration were identified during the course of two independent screenings and named salt resistant (sre) and salobreño (sañ). The sre and sañ mutants also were able to germinate in high-osmoticum medium, indicating that they are osmotolerant in a germination assay. Complementation analyses revealed that sre1-1, sre1-2, sañ3-1, and sañ3-2 were alleles of the abscisic acid (ABA) biosynthesis ABA2 gene. A map-based cloning strategy allowed the identification of the ABA2 gene and molecular characterization of four new aba2 alleles. The ABA2 gene product belongs to the family of short-chain dehydrogenases/reductases, which are known to be NAD- or NADP-dependent oxidoreductases. Recombinant ABA2 protein produced in Escherichia coli exhibits a K m value for xanthoxin of 19 μM and catalyzes in a NAD-dependent manner the conversion of xanthoxin to abscisic aldehyde, as determined by HPLC–mass spectrometry. The ABA2 mRNA is expressed constitutively in all plant organs examined and is not upregulated in response to osmotic stress. The results of this work are discussed in the context of previous genetic and biochemical evidence regarding ABA biosynthesis, confirming the xanthoxin→abscisic aldehyde→ABA transition as the last steps of the major ABA biosynthetic pathway.
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Cites
Genetic control of abscisic acid biosynthesis in maize
Tan, B. C., Schwartz, S. H., Zeevaart, J. A. D., & McCarty, D. R. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.22.12235 · Cited by 629
Cited by
Endo, A., Sawada, Y., Takahashi, H., Okamoto, M., Ikegami, K., Koiwai, H., Seo, M., Toyomasu, T., Mitsuhashi, W., Shinozaki, K., et al. (2008) · Plant Physiology · doi:10.1104/pp.108.116632 · Cited by 369
Revisiting the Basal Role of ABA – Roles Outside of Stress
Yoshida, T., Christmann, A., Yamaguchi-Shinozaki, K., Grill, E., & Fernie, A. R. (2019) · Trends in Plant Science · doi:10.1016/j.tplants.2019.04.008 · Cited by 268
The role of NAD biosynthesis in plant development and stress responses
Hashida, S.-N., Takahashi, H., & Uchimiya, H. (2009) · Annals of Botany · doi:10.1093/aob/mcp019 · Cited by 195
Related enzymes solve evolutionarily recurrent problems in the metabolism of carotenoids
MOISE, A., VONLINTIG, J., & PALCZEWSKI, K. (2005) · Trends in Plant Science · doi:10.1016/j.tplants.2005.02.006 · Cited by 153
Moummou, H., Kallberg, Y., Tonfack, L. B., Persson, B., & van der Rest, B. (2012) · BMC Plant Biology · doi:10.1186/1471-2229-12-219 · Cited by 134
Metadata
- Authors
- González-Guzmán, M., Apostolova, N., Bellés, J. M., Barrero, J. M., Piqueras, P., Ponce, M. R., Micol, J. L., Serrano, R., & Rodríguez, P. L.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-08-01
- Volume
- 14
- Issue
- 8
- Pages
- 1833-1846
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2163183563
- Cited by
- 520
- References
- 1
- FWCI
- 4.21
- Type
- journal article
- Language
- en
- Open access
- bronze
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- González-Guzmán, M., Apostolova, N., Bellés, J. M., Barrero, J. M., Piqueras, P., Ponce, M. R., Micol, J. L., Serrano, R., & Rodríguez, P. L. (2002). The Short-Chain Alcohol Dehydrogenase ABA2 Catalyzes the Conversion of Xanthoxin to Abscisic Aldehyde[W]. The Plant Cell. https://doi.org/10.1105/tpc.002477
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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