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Targeting of Active Sialyltransferase to the Plant Golgi Apparatus

Open access
Wee, Edmund G.-T., Sherrier, D. Janine, Prime, Tracy A., Dupree, Paul·1998·The Plant Cell

Abstract

Glycosyltransferases in the Golgi apparatus synthesize cell wall polysaccharides and elaborate the complex glycans of glycoproteins. To investigate the targeting of this type of enzyme to plant Golgi compartments, we generated transgenic Arabidopsis plants expressing alpha-2,6-sialyltransferase, a glycosyltransferase of the mammalian trans-Golgi cisternae and the trans-Golgi network. Biochemical analysis as well as immunolight and immunoelectron microscopy of these plants indicate that the protein is targeted specifically to the Golgi apparatus. Moreover, the protein is predominantly localized to the cisternae and membranes of the trans side of the organelle. When supplied with the appropriate substrates, the enzyme has significant alpha-2,6-sialyltransferase activity. These results indicate a conservation of glycosyltransferase targeting mechanisms between plant and mammalian cells and also demonstrate that glycosyltransferases can be subcompartmentalized to specific cisternae of the plant Golgi apparatus.

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Metadata

Authors
Wee, E. G.-T., Sherrier, D. J., Prime, T. A., & Dupree, P.
Venue
The Plant Cell
Published
1998-10
Volume
10
Issue
10
Pages
1759-1768
ISSN
  • 1040-4651 (ISSN-L)
  • 1532-298X
OpenAlex
W1989109435
Cited by
181
References
1
FWCI
2.14
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Targeting of Active Sialyltransferase to the Plant Golgi Apparatus. https://app.cochat.ai/papers/10.1105/tpc.10.10.1759
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Wee, E. G.-T., Sherrier, D. J., Prime, T. A., & Dupree, P. (1998). Targeting of Active Sialyltransferase to the Plant Golgi Apparatus. The Plant Cell. https://doi.org/10.1105/tpc.10.10.1759

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