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PIL5, a Phytochrome-Interacting bHLH Protein, Regulates Gibberellin Responsiveness by Binding Directly to the GAI and RGA Promoters in Arabidopsis Seeds
Open accessAbstract
Previous work showed that PHYTOCHROME-INTERACTING FACTOR3-LIKE5 (PIL5), a light-labile basic helix-loop-helix protein, inhibits seed germination by repressing GIBBERELLIN 3β-HYDROXYLASE1 (GA3ox1) and GA3ox2 and activating a gibberellic acid (GA) catabolic gene (GA2ox2). However, we show persistent light-dependent and PIL5-inhibited germination behavior in the absence of both de novo GA biosynthesis and deactivation by GA2ox2, suggesting that PIL5 regulates not only GA metabolism but also GA responsiveness. PIL5 increases the expression of two GA repressor (DELLA) genes, GA-INSENSITIVE (GAI) and REPRESSOR OF GA1-3 (RGA/RGA1), in darkness. The hypersensitivity of gai-t6 rga-28 to red light and the suppression of germination defects of a rga-28 PIL5 overexpression line show the significant role of this transcriptional regulation in seed germination. PIL5 also increases abscisic acid (ABA) levels by activating ABA biosynthetic genes and repressing an ABA catabolic gene. PIL5 binds directly to GAI and RGA promoters but not to GA and ABA metabolic gene promoters. Together, our results show that light signals perceived by phytochromes cause a reduction in the PIL5 protein level, which in turn regulates the transcription of two DELLA genes directly and that of GA and ABA metabolic genes indirectly.
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Citations
Cited by
Molecular Aspects of Seed Dormancy
Finkelstein, R., Reeves, W., Ariizumi, T., & Steber, C. (2008) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.59.032607.092740 · Cited by 1,379
Molecular networks regulating Arabidopsis seed maturation, after‐ripening, dormancy and germination
Holdsworth, M. J., Bentsink, L., & Soppe, W. J. J. (2008) · New Phytologist · doi:10.1111/j.1469-8137.2008.02437.x · Cited by 964
Light-Regulated Plant Growth and Development
Kami, C., Lorrain, S., Hornitschek, P., & Fankhauser, C. (2010) · Current Topics in Developmental Biology · doi:10.1016/s0070-2153(10)91002-8 · Cited by 927
Global Analysis of DELLA Direct Targets in Early Gibberellin Signaling in Arabidopsis
Zentella, R., Zhang, Z.-L., Park, M., Thomas, S. G., Endo, A., Murase, K., Fleet, C. M., Jikumaru, Y., Nambara, E., Kamiya, Y., et al. (2007) · The Plant Cell · doi:10.1105/tpc.107.054999 · Cited by 678
Self-citationDecoding of Light Signals by Plant Phytochromes and Their Interacting Proteins
Bae, G., & Choi, G. (2008) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.59.032607.092859 · Cited by 490
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Metadata
- Authors
- Oh, E., Yamaguchi, S., Hu, J., Yusuke, J., Jung, B., Paik, I., Lee, H.-S., Sun, T.-P., Kamiya, Y., & Choi, G.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-04-01
- Volume
- 19
- Issue
- 4
- Pages
- 1192-1208
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2122032389
- Cited by
- 477
- References
- 0
- FWCI
- 33.11
- Type
- journal article
- Language
- en
- Open access
- bronze
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- The paper
- Oh, E., Yamaguchi, S., Hu, J., Yusuke, J., Jung, B., Paik, I., Lee, H.-S., Sun, T.-P., Kamiya, Y., & Choi, G. (2007). PIL5, a Phytochrome-Interacting bHLH Protein, Regulates Gibberellin Responsiveness by Binding Directly to the GAI and RGA Promoters in Arabidopsis Seeds. The Plant Cell. https://doi.org/10.1105/tpc.107.050153
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