The DOI matches its Crossref record; 68 of the 68 references it cites were found in a registry or an index.
Structural Evidence for the Evolution of Xyloglucanase Activity from Xyloglucan Endo -Transglycosylases: Biological Implications for Cell Wall Metabolism
Open accessAbstract
High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure–function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo-transglycosylase Ptt-XET16-34 from hybrid aspen (Populus tremula × Populus tremuloides). Production of the loop deletion variant Tm-NXG1-ΔYNIIG yielded an enzyme that was structurally similar to Ptt-XET16-34 and had a greatly increased transglycosylation:hydrolysis ratio. Comprehensive bioinformatic analyses of XTH gene products, together with detailed kinetic data, strongly suggest that xyloglucanase activity has evolved as a gain of function in an ancestral GH16 XET to meet specific biological requirements during seed germination, fruit ripening, and rapid wall expansion.
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Evolution and Diversity of Plant Cell Walls: From Algae to Flowering Plants
Popper, Z. A., Michel, G., Hervé, C., Domozych, D. S., Willats, W. G. T., Tuohy, M. G., Kloareg, B., & Stengel, D. B. (2011) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042110-103809 · Cited by 732
Self-citationHehemann, J.-H., Kelly, A. G., Pudlo, N. A., Martens, E. C., & Boraston, A. B. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1211002109 · Cited by 338
Metadata
- Authors
- Baumann, M. J., Eklöf, J. M., Michel, G., Kallas, A. M., Teeri, T. T., Czjzek, M., & Brumer, H.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-06-01
- Volume
- 19
- Issue
- 6
- Pages
- 1947-1963
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2135616804
- Cited by
- 288
- References
- 68
- FWCI
- 23.49
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Baumann, M. J., Eklöf, J. M., Michel, G., Kallas, A. M., Teeri, T. T., Czjzek, M., & Brumer, H. (2007). Structural Evidence for the Evolution of Xyloglucanase Activity from Xyloglucan Endo -Transglycosylases: Biological Implications for Cell Wall Metabolism. The Plant Cell. https://doi.org/10.1105/tpc.107.051391
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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