The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cytochrome P450 Family Member CYP704B2 Catalyzes the ω -Hydroxylation of Fatty Acids and Is Required for Anther Cutin Biosynthesis and Pollen Exine Formation in Rice
Open accessAbstract
The anther cuticle and microspore exine act as protective barriers for the male gametophyte and pollen grain, but relatively little is known about the mechanisms underlying the biosynthesis of the monomers of which they are composed. We report here the isolation and characterization of a rice (Oryza sativa) male sterile mutant, cyp704B2, which exhibits a swollen sporophytic tapetal layer, aborted pollen grains without detectable exine, and undeveloped anther cuticle. In addition, chemical composition analysis indicated that cutin monomers were hardly detectable in the cyp704B2 anthers. These defects are caused by a mutation in a cytochrome P450 family gene, CYP704B2. The CYP704B2 transcript is specifically detected in the tapetum and the microspore from stage 8 of anther development to stage 10. Heterologous expression of CYP704B2 in yeast demonstrated that CYP704B2 catalyzes the production of ω -hydroxylated fatty acids with 16 and 18 carbon chains. Our results provide insights into the biosynthesis of the two biopolymers sporopollenin and cutin. Specifically, our study indicates that the ω -hydroxylation pathway of fatty acids relying on this ancient CYP704B family, conserved from moss to angiosperms, is essential for the formation of both cuticle and exine during plant male reproductive and spore development.
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Cites
The Carbon Monoxide-binding Pigment of Liver Microsomes
Omura, T., & Sato, R. (1964) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)82244-3 · Cited by 11,781
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A P450‐centric view of plant evolution
Nelson, D., & Werck‐Reichhart, D. (2011) · The Plant Journal · doi:10.1111/j.1365-313x.2011.04529.x · Cited by 720
Self-citationGenetic and Biochemical Mechanisms of Pollen Wall Development
Shi, J., Cui, M., Yang, L., Kim, Y.-J., & Zhang, D. (2015) · Trends in Plant Science · doi:10.1016/j.tplants.2015.07.010 · Cited by 422
Self-citationThe cytochrome P450 superfamily: Key players in plant development and defense
XU, J., WANG, X.-Y., & GUO, W.-Z. (2015) · Journal of Integrative Agriculture · doi:10.1016/s2095-3119(14)60980-1 · Cited by 355
A High-Density SNP Genotyping Array for Rice Biology and Molecular Breeding
Chen, H., Xie, W., He, H., Yu, H., Chen, W., Li, J., Yu, R., Yao, Y., Zhang, W., He, Y., et al. (2014) · Molecular Plant · doi:10.1093/mp/sst135 · Cited by 326
Rice MADS3 Regulates ROS Homeostasis during Late Anther Development
Hu, L., Liang, W., Yin, C., Cui, X., Zong, J., Wang, X., Hu, J., & Zhang, D. (2011) · The Plant Cell · doi:10.1105/tpc.110.074369 · Cited by 326
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Metadata
- Authors
- Li, H., Pinot, F., Sauveplane, V., Werck-Reichhart, D., Diehl, P., Schreiber, L., Franke, R., Zhang, P., Chen, L., Gao, Y., Liang, W., & Zhang, D.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-01-01
- Volume
- 22
- Issue
- 1
- Pages
- 173-190
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2137358559
- Cited by
- 374
- References
- 1
- FWCI
- 7.92
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Li, H., Pinot, F., Sauveplane, V., Werck-Reichhart, D., Diehl, P., Schreiber, L., Franke, R., Zhang, P., Chen, L., Gao, Y., Liang, W., & Zhang, D. (2010). Cytochrome P450 Family Member CYP704B2 Catalyzes the ω -Hydroxylation of Fatty Acids and Is Required for Anther Cutin Biosynthesis and Pollen Exine Formation in Rice. The Plant Cell. https://doi.org/10.1105/tpc.109.070326
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