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Indole Glucosinolate Biosynthesis Limits Phenylpropanoid Accumulation in Arabidopsis thaliana
Open accessAbstract
Plants produce an array of metabolites (including lignin monomers and soluble UV-protective metabolites) from phenylalanine through the phenylpropanoid biosynthetic pathway. A subset of plants, including many related to Arabidopsis thaliana, synthesizes glucosinolates, nitrogen- and sulfur-containing secondary metabolites that serve as components of a plant defense system that deters herbivores and pathogens. Here, we report that the Arabidopsis thaliana reduced epidermal fluorescence5 (ref5-1) mutant, identified in a screen for plants with defects in soluble phenylpropanoid accumulation, has a missense mutation in CYP83B1 and displays defects in glucosinolate biosynthesis and in phenylpropanoid accumulation. CYP79B2 and CYP79B3 are responsible for the production of the CYP83B1 substrate indole-3-acetaldoxime (IAOx), and we found that the phenylpropanoid content of cyp79b2 cyp79b3 and ref5-1 cyp79b2 cyp79b3 plants is increased compared with the wild type. These data suggest that levels of IAOx or a subsequent metabolite negatively influence phenylpropanoid accumulation in ref5 and more importantly that this crosstalk is relevant in the wild type. Additional biochemical and genetic evidence indicates that this inhibition impacts the early steps of the phenylpropanoid biosynthetic pathway and restoration of phenylpropanoid accumulation in a ref5-1 med5a/b triple mutant suggests that the function of the Mediator complex is required for the crosstalk.
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Aldoximes, which accumulate in CYP83B1 mutants, increase KFB-mediated PAL degradation through MED5 transcriptional regulation as well as other unknown mechanisms.
“The model emerging from these studies is that aldoximes, which accumulate in CYP83B1 mutants, increase KFB-mediated PAL degradation through MED5 transcriptional regulation as well as other, yet unknown, mechanisms (Kim et al., 2015;Kim et al., 2020).”
From Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional Trichotomy (2020) · cites this paper as Kim et al., 2015
The section on phenylpropanoid/glucosinolate crosstalk (page unavailable) proposes that aldoxime accumulation in ref5/CYP83B1 mutants reduces PAL activity through MED5-dependent transcriptional regulation. The section on altered aldoxime levels (page unavailable) says other mechanisms are unclear and suggests ubiquitin-mediated PAL turnover as a possibility; it does not establish increased KFB-mediated degradation or link KFBs to MED5. (LLM, conf=0.89)
Experimental — accuracy not yet measuredGlucosinolates and benzoxazinoids also seem to regulate the accumulation of other secondary metabolites.
“Interestingly, glucosinolates and benzoxazinoids also seem to regulate the accumulation of other secondary metabolites (Hemm et al., 2003;Kim et al., 2015;Li et al., 2018a).”
From Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional Trichotomy (2020) · cites this paper as Kim et al., 2015
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Erb, M., & Kliebenstein, D. J. (2020) · Plant Physiology · doi:10.1104/pp.20.00433 · Cited by 1,333
Metadata
- Authors
- Kim, J. I., Dolan, W. L., Anderson, N. A., & Chapple, C.
- Affiliations
- Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907
- Purdue University West Lafayette (US)
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-05
- Volume
- 27
- Issue
- 5
- Pages
- 1529-1546
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- PMC
- PMC4456644
- OpenAlex
- W2173743066
- Cited by
- 117
- References
- 0
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 9 · 2023: 10 · 2022: 12 · 2021: 15 · 2020: 17 · 2019: 7 · 2018: 7 · 2017: 9 · 2016: 10 · 2015: 5
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Plant Cell (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- U.S. Department of Energy (DESC0000997, DEFG0207ER15905)
- Purdue University
- Office of Science (DESC0000997)
- Basic Energy Sciences (DE-SC0000997, SC0000997)
- Energy Frontier Research Centers
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- indole glucosinolates, phenylpropanoid biosynthesis, indole-3-acetaldoxime, CYP79B2, Mediator complex, lignin monomers, Arabidopsis thaliana
- MeSH terms
- Aldehyde Dehydrogenase, Cytochrome P-450 Enzyme System, Glucosinolates, Indoleacetic Acids, Indoles, Lignin, Membrane Proteins, Oximes, Plant Growth Regulators, Arabidopsis, Gene Expression Regulation, Plant, Propanols, Mutation, Missense, Arabidopsis Proteins, Plants, Genetically Modified, Seedlings, Biosynthetic Pathways
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