The DOI matches its Crossref record.
An approach to rapid protein crystallization using nanodroplets
Abstract
An approach that enables up to a two order of magnitude reduction in the amount of protein required and a tenfold reduction in the amount of time required for vapor-diffusion protein crystallization is reported. A prototype high-throughput automated system was used for the production of diffraction-quality crystals for a variety of proteins from a screen of 480 conditions using drop volumes as small as 20 nL. This approach results in a significant reduction in the time and cost of protein structure determination, and allows for larger and more efficient screens of crystallization parameter space. The ability to produce diffraction-quality crystals rapidly with minimal quantities of protein enables high-throughput efforts in structural genomics and structure-based drug discovery.
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Cited by
Zheng, B., Tice, J. D., Roach, L. S., & Ismagilov, R. F. (2004) · Angewandte Chemie International Edition · doi:10.1002/anie.200453974 · Cited by 398
Das, D., Kozbial, P., Han, G. W., Carlton, D., Jaroszewski, L., Abdubek, P., Astakhova, T., Axelrod, H. L., Bakolitsa, C., Chen, C., et al. (2010) · Acta Crystallographica Section F Structural Biology and Crystallization Communications · doi:10.1107/s1744309109018168 · Cited by 2
Metadata
- Authors
- Santarsiero, B. D., Yegian, D. T., Lee, C. C., Spraggon, G., Gu, J., Scheibe, D., Uber, D. C., Cornell, E. W., Nordmeyer, R. A., Kolbe, W. F., Jin, J., Jones, A. L., Jaklevic, J. M., Schultz, P. G., & Stevens, R. C.
- Venue
- Journal of Applied Crystallography
- Published
- 2002-03-22
- Volume
- 35
- Issue
- 2
- Pages
- 278-281
- ISSN
- 0021-8898 (ISSN-L)
- OpenAlex
- W2094504398
- Cited by
- 240
- References
- 0
- FWCI
- 18.25
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: An approach to rapid protein crystallization using nanodroplets. https://app.cochat.ai/papers/10.1107/s0021889802001474
- The paper
- Santarsiero, B. D., Yegian, D. T., Lee, C. C., Spraggon, G., Gu, J., Scheibe, D., Uber, D. C., Cornell, E. W., Nordmeyer, R. A., Kolbe, W. F., Jin, J., Jones, A. L., Jaklevic, J. M., Schultz, P. G., & Stevens, R. C. (2002). An approach to rapid protein crystallization using nanodroplets. Journal of Applied Crystallography. https://doi.org/10.1107/s0021889802001474
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
