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His-tag impact on structure
Abstract
Crystallographers are increasingly determining structures of protein constructs that include His tags. Many have taken for granted that these tags have little effect on the native structure. This paper surveys and compares crystal structures with and without His tags. It is observed that actual refined tag residues fitted into density occur in less that 10% of the tagged sequences. However, higher resolution crystals are observed when this occurs. It is shown that these purification tags generally have no significant effect on the structure of the native protein. Resolution and R factors are not affected, but the overall B factors are slightly higher. Additional annotation in the PDB format to make tag definition explicit is suggested.
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Cited by
Recombinant protein expression in Escherichia coli: advances and challenges
Rosano, G. L., & Ceccarelli, E. A. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00172 · Cited by 2,556
Protein production and purification
Structural Genomics Consortium, Biologiques, A. E. F. D. M., Berkeley Structural Genomics Center, China Structural Genomics Consortium, Integrated Center for Structure and Function Innovation, Israel Structural Proteomics Center, Joint Center for Structural Genomics, Midwest Center for Structural Genomics, New York Structural GenomiX Research Center for Structural Genomics, Northeast Structural Genomics Consortium, et al. (2008) · Nature Methods · doi:10.1038/nmeth.f.202 · Cited by 916
Metadata
- Authors
- Carson, M., Johnson, D. H., McDonald, H., Brouillette, C., & DeLucas, L. J.
- Venue
- Acta Crystallographica Section D Biological Crystallography
- Published
- 2007-02-21
- Volume
- 63
- Issue
- 3
- Pages
- 295-301
- ISSN
- 0907-4449 (ISSN-L)
- OpenAlex
- W2076070614
- Cited by
- 240
- References
- 0
- FWCI
- 4.75
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: His-tag impact on structure. https://app.cochat.ai/papers/10.1107/s0907444906052024
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- Carson, M., Johnson, D. H., McDonald, H., Brouillette, C., & DeLucas, L. J. (2007). His-tag impact on structure. Acta Crystallographica Section D Biological Crystallography. https://doi.org/10.1107/s0907444906052024
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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