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Cited by 1,814

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ChloroP, a neural network‐based method for predicting chloroplast transit peptides and their cleavage sites

Open access
Emanuelsson, Olof, Nielsen, Henrik, Heijne, Gunnar Von·1999·Protein Science

Abstract

We present a neural network based method (ChloroP) for identifying chloroplast transit peptides and their cleavage sites. Using cross‐validation, 88% of the sequences in our homology reduced training set were correctly classified as transit peptides or nontransit peptides. This performance level is well above that of the publicly available chloroplast localization predictor PSORT. Cleavage sites are predicted using a scoring matrix derived by an automatic motif‐finding algorithm. Approximately 60% of the known cleavage sites in our sequence collection were predicted to within 62 residues from the cleavage sites given in SWISS‐PROT. An analysis of 715 Arabidopsis thaliana sequences from SWISS‐PROT suggests that the ChloroP method should be useful for the identification of putative transit peptides in genome‐wide sequence data. The ChloroP predictor is available as a web‐server at http://www.cbs.dtu.dk/services/ChloroP/.

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Metadata

Authors
Emanuelsson, O., Nielsen, H., & Heijne, G. V.
Venue
Protein Science
Publisher
Wiley
Published
1999-01
Volume
8
Issue
5
Pages
978-984
ISSN
  • 0961-8368 (ISSN-L)
  • 1469-896X
OpenAlex
W2129095580
Cited by
1,814
References
2
FWCI
17.07
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: ChloroP, a neural network‐based method for predicting chloroplast transit peptides and their cleavage sites. https://app.cochat.ai/papers/10.1110/ps.8.5.978
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Emanuelsson, O., Nielsen, H., & Heijne, G. V. (1999). ChloroP, a neural network‐based method for predicting chloroplast transit peptides and their cleavage sites. Protein Science. https://doi.org/10.1110/ps.8.5.978

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