CoChat
Sign Up
Cited by 45Redox biology and oxidative stressGlutathione Transferases and PolymorphismsAntioxidant Activity and Oxidative Stress

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Inactivation of Enzymes of the Glutathione Antioxidant System by Treatment of Cultured Human Keratinocytes with Peroxides

Open access
Vessey, Donald A., Lee, Kyung-Hee·1993·Journal of Investigative Dermatology
DOI 10.1111/1523-1747.ep12476735
Open this paper in CoChat — free account

Abstract

Either metal ions, H2O2, t-butyl hydroperoxide (tBHP), or cumene hydroperoxide (CHP) was added to the medium of cultured human keratinocytes, and the activities of key peroxide-metabolizing enzymes were examined in a sonicated cell supernatant from the treated cells. 200 microM Fe++ +200 microM Fe was without effect on any enzyme activity. 700 microM CHP or tBHP decreased glutathione (GSH) peroxidase activity by 90% after 5 h and by 100% at 20 h, even if the CHP or tBHP was removed from the media after 90 min. H2O2 at 700 microM caused a brief 17% decrease in activity, which was followed by complete recovery. GSH peroxidase was found to be rapidly inactivated in vitro by CHP, but the enzyme was also inactivated at 37 degrees C even in the absence of CHP. GSH prevented both types of inactivation. Consistent with this in vitro data, in vivo depletion of the GSH pool with buthionine sulfoximine led to lower levels of GSH peroxidase and increased sensitivity to peroxide-induced inactivation. Neither GSH reductase nor GSH S-transferase were inactivated by any treatment although CHP did cause a small increase in the activity of the latter, which was not due to induction. The activity of glucose-6-phosphate dehydrogenase was decreased 50% following treatment for 5 h with 700 microM CHP or tBHP, whereas H2O2 treatment caused a brief 15% decline, followed by recovery. The effects of peroxides were not altered by changing the concentration of Ca++ in the media. Catalase was unaffected by concentrations of peroxide up to 700 microM. Inhibition of catalase with aminotriazole slightly enhanced the toxicity of 700 microns H2O2. In summary, organic hydroperoxides at relatively low concentrations inactive key enzymes of the glutathione pathway, but hydrogen peroxide does not.

Overview

Integrity signals

Registry

Crossref · 10.1111/1523-1747.ep12476735

Cites

29 of 29 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Cited by

Sorted by citations

See all 45

Related

See all

Metadata

Authors
Vessey, D. A., & Lee, K.-H.
Affiliations
San Francisco VA Medical Center (US)
Venue
Journal of Investigative Dermatology
Publisher
Elsevier BV
Published
1993-06
Volume
100
Issue
6
Pages
829-833
ISSN
0022-202X (ISSN-L)
DOI
10.1111/1523-1747.ep12476735
OpenAlex
W2009191072
Cited by
45
References
29
Citations by year
2025: 1 · 2021: 1 · 2020: 1 · 2017: 1 · 2014: 2 · 2013: 3 · 2012: 2
FWCI
0.77
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Investigative Dermatology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR39448, P01AR039448)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
  • Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
Keywords
glutathione peroxidase, enzyme inactivation, cumene hydroperoxide, tert-butyl hydroperoxide, hydrogen peroxide, keratinocytes, glutathione pathway, GSH reductase, glucose-6-phosphate dehydrogenase, catalase, buthionine sulfoximine, oxidative stress, 3-amino-1,2,4-triazole, metal ions
MeSH terms
Antimetabolites, Benzene Derivatives, Calcium, Catalase, Cells, Cultured, Culture Media, Enzyme Activation, Ferric Compounds, Ferrous Compounds, Glucosephosphate Dehydrogenase, Glutathione, Glutathione Reductase, Humans, Hydrogen Peroxide, Methionine Sulfoximine, Peroxides, Time Factors, Keratinocytes, Oxidants, Buthionine Sulfoximine, tert-Butylhydroperoxide

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review