Variation in the hindgut microbial communities of the Florida manatee,Trichechus manatus latirostris over winter in Crystal River, Florida
Open accessAbstract
The Florida manatee, Trichechus manatus latirostris, is a hindgut-fermenting herbivore. In winter, manatees migrate to warm water overwintering sites where they undergo dietary shifts and may suffer from cold-induced stress. Given these seasonally induced changes in diet, the present study aimed to examine variation in the hindgut bacterial communities of wild manatees overwintering at Crystal River, west Florida. Faeces were sampled from 36 manatees of known sex and body size in early winter when manatees were newly arrived and then in mid-winter and late winter when diet had probably changed and environmental stress may have increased. Concentrations of faecal cortisol metabolite, an indicator of a stress response, were measured by enzyme immunoassay. Using 454-pyrosequencing, 2027 bacterial operational taxonomic units were identified in manatee faeces following amplicon pyrosequencing of the 16S rRNA gene V3/V4 region. Classified sequences were assigned to eight previously described bacterial phyla; only 0.36% of sequences could not be classified to phylum level. Five core phyla were identified in all samples. The majority (96.8%) of sequences were classified as Firmicutes (77.3 ± 11.1% of total sequences) or Bacteroidetes (19.5 ± 10.6%). Alpha-diversity measures trended towards higher diversity of hindgut microbiota in manatees in mid-winter compared to early and late winter. Beta-diversity measures, analysed through PERMANOVA, also indicated significant differences in bacterial communities based on the season.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Clostridium butyricum was noted to be one of the 10 most abundant bacterial operational taxonomic units in fecal samples from Crystal River manatees.
“Clostridium butyricum was noted to be one of the 10 most abundant bacterial operational taxonomic units in fecal samples from Crystal River manatees (Merson et al., 2014).”
From Novel Lethal Clostridial Infection in Florida Manatees (Trichechus manatus latirostris): Cause of the 2013 Unusual Mortality Event in the Indian River Lagoon (2022) · cites this paper as Merson et al., 2014
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
MedlineRanker: flexible ranking of biomedical literature
Fontaine, J.-F., Barbosa-Silva, A., Schaefer, M., Huska, M. R., Muro, E. M., & Andrade-Navarro, M. A. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp353 · Cited by 147 · see citing works
Flagged first, then by citations
Research works printed but not linked: 8
- Burgess EA, Brown JL & Lanyon JM (2013) Sex, scarring, and stress: understanding seasonal costs in a cryptic marine mammal. Conserv Physiol 1: Article ID cot014. DOI: 10. 1093/conphys/cot014. · DOI
- Deutsch CJ, Edwards HH & Barlas ME (2006) Florida manatee foraging behavior around a winter warm-water refuge. Final Report to the Wildlife Foundation of Florida. Grant No. CWT0405-12. FWC/FWRI file FCWT0405-12-F. pp. 42. Dierenfeld ES (1997) Captive wild animal nutrition: a historical perspective. Proc Nutr Soc 5: 989-999.
- Lomolino MV (1977) The ecological role of the Florida manatee Trichechus manatus latirostris in water hyacinth dominated ecosystems. MS Thesis, University of Florida, Gainesville, FA.
- Nabor P & Patton GW (1989) Aerial studies of the West Indian manatee (Trichechus manatus) from Anna Maria Florida to Northern Charlotte Harbor including the Myakka River: recommended habitat protection and manatee management strategies. Mote Marine Laboratory Technical Report no 134: pp. 94. County of Sarasota, Florida.
- Reep RL & Bonde RK (2006) The Florida Manatee Biology and Conservation. pp. 189. University Press of Florida, Gainesville, FL.
- Reid JP, Bonde RK & O'Shea TJ (1995) Reproduction and mortality of radio-tagged and recognizable manatees on the Atlantic coast of Florida. Population Biology of the Florida Manatee. Information and Technology Report 1. (O'Shea TJ, Ackerman BB & Percival HF, eds), pp. 171-191. National Biological Service, Washington, D.C. Ren N, Xing D, Rittmann BE, Zhao L, Xie T & Zhao X (2007) Microbial community structure of ethanol type fermentation in bio-hydrogen production. Environ Microbiol 9: 1112-1125.
- Reynolds JE III (1980) Aspects of the structural and functional anatomy of the gastrointestinal tract of the West Indian manatee, Trichechus manatus. PhD Thesis, University of Miami, Miami, FA, USA.
- Yu Z & Forster RJ (2005) Nucleic acid extraction, oligonucleotide probes and PCR methods. Methods in Gut Microbial Ecology for Ruminants (Makkar HPS, & McSweeney CS, eds), pp 81-104. Springer, the Netherlands.
Cited by
Sorted by citations
Landsberg, J. H., Tabuchi, M., Rotstein, D. S., Subramaniam, K., Rodrigues, T. C. S., Waltzek, T. B., Stacy, N. I., Wilson, P. W., Kiryu, Y., Uzal, F. A., et al. (2022) · Frontiers in Marine Science · doi:10.3389/fmars.2022.841857 · Cited by 20 · see citing works
“…the Crystal River (on Florida’s west coast) and eastern Florida Atlantic (Mao et al., 2011, cited in Frontiers in Marine Science | www.frontiersin.org 15 March 2022 | Volume 9 | Article 841857 Merson et al., 2014), but in-depth analysis of clostridial diversity and speciation has not been done.”
Background
- Authors
- Merson, S. D., Ouwerkerk, D., Gulino, L.-M., Klieve, A., Bonde, R. K., Burgess, E. A., & Lanyon, J. M.
- Affiliations
- The University of Queensland (AU)
- United States Geological Survey (US)
- Corresponding author
- Lanyon, Janet M.
- Venue
- FEMS Microbiology Ecology
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-12-09
- Volume
- 87
- Issue
- 3
- Pages
- 601-615
- ISSN
- 0168-6496 (ISSN-L)
- OpenAlex
- W2092931984
- Cited by
- 33
- References
- 1
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 4 · 2019: 10 · 2018: 2 · 2017: 4 · 2016: 2 · 2015: 3
- FWCI
- 0.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Ecology (publishedVersion)
- PubMed (publishedVersion)
- Queensland Department of Agriculture and Fisheries archive of scientific and research publications (Queensland Department of Agriculture and Fisheries) (submittedVersion)
- Funders
- U.S. Fish and Wildlife Service
- U.S. Geological Survey
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Physical Sciences › Environmental Science › Ecology › Marine animal studies overview
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Keywords
- Florida manatee, Trichechus manatus latirostris, hindgut microbiota, gut microbiota, seasonal variation, overwintering, dietary change, cold stress, faecal cortisol metabolites, alpha diversity, beta diversity, Firmicutes, Bacteroidetes, herbivorous mammals, Crystal River
- MeSH terms
- Animals, Bacteria, Diet, Digestive System, DNA, Bacterial, Feces, Female, Florida, Male, RNA, Ribosomal, 16S, Seasons, Bacterial Typing Techniques, Sequence Analysis, DNA, Trichechus manatus, Biodiversity, Rivers
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
