Long non‐coding RNA TPT1‐AS1 inhibits ferroptosis in ovarian cancer by regulating GPX4 via CREB1 regulation
Abstract
Background Long non-coding RNAs (lncRNAs) play crucial roles in cellular processes, with dysregulation implicated in various diseases, including cancers. The lncRNA TPT1-AS1 (TPT1 Antisense RNA 1) promotes tumor progression in several cancers, including ovarian cancer (OC), but its influence on ferroptosis and interaction with other proteins remains underexplored. Methods In this study, we employed a multi-faceted approach to investigate the functional significance of TPT1-AS1 in OC. We assessed TPT1-AS1 expression in OC specimens and cell lines using RT-qPCR, in situ hybridization (ISH), and fluorescence in situ hybridization (FISH) assays. Functional assays included evaluating the impact of TPT1-AS1 knockdown on OC cell proliferation, migration, invasiveness, and cell cycle progression. Further, we explored and validated the interaction of TPT1-AS1 with other proteins using bioinformatics. Finally, we investigated TPT1-AS1 involvement in erastin-induced ferroptosis using Iron Assay, Malondialdehyde (MDA) assay, and reactive oxygen species (ROS) detection. Results Our findings revealed that TPT1-AS1 overexpression in OC correlated with an unfavorable prognosis. TPT1-AS1 knockdown suppressed cell proliferation, migration, and invasiveness. Additionally, TPT1-AS1 inhibited erastin-induced ferroptosis, and in vivo experiments confirmed its oncogenic impact on tumor development. Mechanistically, TPT1-AS1 was found to regulate Glutathione Peroxidase 4 (GPX4) transcription via CRE…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Several transcription factors including AP-1, CREB, ATF4, and NRF2 have been shown to regulate either directly or indirectly GPX4 levels in different cellular contexts.
“Several transcription factors including AP-1 (60, 61), CREB (62, 63), ATF4 (64), and NRF2 (45, 65, 66) have been shown to regulate either directly or indirectly GPX4 levels in different cellular contexts.”
From Secreted Apoe rewires melanoma cell state vulnerability to ferroptosis (2024) · cites this paper as [63]
The citation lists haven’t been fetched yet
References 63 · Cited by 7
- Authors
- Cao, L., Wang, Y., Liu, J., Bai, X., & Chi, X.
- Affiliations
- Xi'an Medical University (CN)
- Shandong First Medical University (CN)
- Corresponding author
- Chi, Xiaohong
- Venue
- American Journal of Reproductive Immunology
- Publisher
- Wiley
- Published
- 2024-08
- Volume
- 92
- Issue
- 2
- Pages
- e13864-e13864
- ISSN
- 0271-7352 (ISSN-L)
- 1046-7408
- 1600-0897
- OpenAlex
- W4401577208
- Cited by
- 7
- References
- 63
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1
- FWCI
- 2.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Reproductive Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Ferroptosis and cancer prognosis
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- long non-coding RNA, ovarian cancer, ferroptosis, GPX4, CREB1, RNA-binding proteins, erastin, tumor progression, cell proliferation and invasion, prognosis
- MeSH terms
- Ferroptosis, Phospholipid Hydroperoxide Glutathione Peroxidase, Tumor Protein, Translationally-Controlled 1, Animals, Cell Movement, Female, Humans, Mice, Nude, Ovarian Neoplasms, Gene Expression Regulation, Neoplastic, Cyclic AMP Response Element-Binding Protein, Cell Line, Tumor, Cell Proliferation, Mice, RNA, Long Noncoding
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
