The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
GENETIC TRANSFORMATION OF THE DIATOMS CYCLOTELLA CRYPTICA AND NAVICULA SAPROPHILA
Abstract
Two species of diatoms were genetically transformed by introducing plasmid vectors containing the Escherichia coli neomycin phosphotransferase II (npt II)gene. Expression of the bacterial npt II gene in the diatoms was achieved using the putative promoter and terminator sequences from the acetyl‐CoA carboxylase gene from the centric diatom Cyclotella cryptica T13L Reimann, Lewin, and Guillard. The vectors were introduced into C. cryptica and the pennate diatom Navicula saprophila NAVIC1 Lange‐Bertalot and Bonik by microprojectile bombardment. Putative transformants were selected based on their ability to grow in the presence of the antibiotic G418, and production of the neomycin phosphotransferase protein by the transformed cells was confirmed by western blotting. The foreign DNA integrated into one or more random sites within the genome of the transformed algal cells, often in the form of tandem repeats. This is the first report of reproducible, stable genetic transformation of a chlorophyll c‐containing alga.
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“Whole-genome sequences have been released for two species, Thalassiosira pseudonana and Phaeodactylum tricornutum, and recent work has shown stable nuclear transformation of these two species and a few other diatoms [91–94].”
From Biofuels from algae: Challenges and potential (2010) · cites this paper as [92]
Citations
Cites
Rapid isolation of high molecular weight plant DNA
Murray, M. G., & Thompson, W. F. (1980) · Nucleic Acids Research · doi:10.1093/nar/8.19.4321 · Cited by 12,156
[1] Production of single-stranded plasmid DNA
Vieira, J., & Messing, J. W. (1987) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(87)53044-0 · Cited by 2,558
Global climate change and terrestrial net primary production
Melillo, J. M., McGuire, A. D., Kicklighter, D. W., Moore, B., Vörösmarty, C. J., & Schloss, A. L. (1993) · Nature · doi:10.1038/363234a0 · Cited by 1,955
The Silica Balance in the World Ocean: A Reestimate
Tréguer, P. J., Nelson, D. M., Bennekom, A. J. V., DeMaster, D. J., Leynaert, A., & Quéguiner, B. (1995) · Science · doi:10.1126/science.268.5209.375 · Cited by 1,489
High-frequency nuclear transformation of Chlamydomonas reinhardtii.
Kindle, K. L. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.3.1228 · Cited by 1,097
Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants
Ow, D. W., Wood, K. V., DeLuca, M. A., de Wet, J. R., Helinski, D. R., & Howell, S. H. (1986) · Science · doi:10.1126/science.234.4778.856 · Cited by 725
[36] Optimizing the biolistic process for different biological applications
Sanford, J. C., Smith, F. D., & Russell, J. A. (1993) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(93)17086-k · Cited by 643
Stable transformation of maize after gene transfer by electroporation
Fromm, M. E., Taylor, L. P., & Walbot, V. (1986) · Nature · doi:10.1038/319791a0 · Cited by 639
Goldschmidt‐Clermont, M. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.15.4083 · Cited by 473
Fincham, J. R. (1989) · Microbiological Reviews · doi:10.1128/mr.53.1.148-170.1989 · Cited by 448
Research works printed but not linked: 3
- Butanaev A. M., 1994, Hygromycin phosphotransferase gene as a dominant selective marker for transformation of Chlamydomonas reinhardtii, Mol. Biol., 28, 682
- Cheney D. P., 1990, Economically Important Marine Plants of the Atlantic: Their Ecology and Cultivation, 15
- Kurtzman A. L., 1991, Direct gene transfer and transient gene expression in a marine red alga using the biolistic method, J. Phycol., 27, 42
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Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745
Self-citationGenetic Engineering of Algae for Enhanced Biofuel Production
Radakovits, R., Jinkerson, R. E., Darzins, A., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00364-09 · Cited by 1,142
SILICON METABOLISM IN DIATOMS: IMPLICATIONS FOR GROWTH
Martin‐Jézéquel, V., Hildebrand, M., & Brzezinski, M. A. (2000) · Journal of Phycology · doi:10.1046/j.1529-8817.2000.00019.x · Cited by 924
High lipid induction in microalgae for biodiesel production
Sharma, K. K., Schuhmann, H., & Schenk, P. M. (2012) · Energies · doi:10.3390/en5051532 · Cited by 886
Biofuels from algae: Challenges and potential
Hannon, M., Gimpel, J., Tran, M., Rasala, B., & Mayfield, S. (2010) · Biofuels · doi:10.4155/bfs.10.44 · Cited by 813
Metadata
- Authors
- Dunahay, T. G., Jarvis, E. E., & Roessler, P. G.
- Affiliations
- National Laboratory of the Rockies (US)
- Venue
- Journal of Phycology
- Publisher
- Wiley
- Published
- 1995-12
- Volume
- 31
- Issue
- 6
- Pages
- 1004-1012
- ISSN
- 0022-3646 (ISSN-L)
- 1529-8817
- OpenAlex
- W2062892053
- Cited by
- 306
- References
- 35
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 7 · 2023: 11 · 2022: 14 · 2021: 12 · 2020: 8 · 2019: 20 · 2018: 12 · 2017: 27 · 2016: 20 · 2015: 20 · 2014: 17 · 2013: 17 · 2012: 19
- FWCI
- 3.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Phycology (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Diatoms and Algae Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Protist diversity and phylogeny
- Keywords
- Cyclotella cryptica, neomycin phosphotransferase II, plasmid vectors, particle bombardment, G418 selection, tandem repeats, foreign DNA integration
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