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Cited by 121melanin and skin pigmentationMelanoma and MAPK PathwaysDermatologic Treatments and Research

Narrow-band ultraviolet-B stimulates proliferation and migration of cultured melanocytes

Wu, Ching‐Shuang, Yu, Chia‐Li, Wu, Chieh‐Shan, Lan, Cheng‐Che E., Yu, Hsin‐Su·2004·Experimental Dermatology
DOI 10.1111/j.0906-6705.2004.00221.x
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Abstract

Abstract: Narrow‐band ultraviolet‐B (UVB) radiation is an effective treatment for vitiligo vulgaris. However, the mechanisms of narrow‐band UVB in inducing repigmentation of vitiligo lesions are not thoroughly clarified. The purpose of our study was to investigate the effects of narrow‐band UVB irradiation on melanocyte proliferation and migration in vitro. Our results showed that the cell counts as well as [3H]thymidine uptake of melanocytes were significantly enhanced by narrow‐band UVB‐irradiated keratinocyte supernatants. In these supernatants, a significant increase in basic fibroblast growth factor (bFGF) and in endothelin‐1 (ET‐1) release was observed. bFGF is a natural mitogen for melanocytes, whereas ET‐1 can stimulate DNA synthesis in melanocytes. This stimulatory effect of melanocyte proliferation by supernatants derived from narrow‐band UVB‐irradiated keratinocytes was significantly reduced by a selective endothelin‐B (ET‐B) receptor antagonist (BQ788), suggesting an essential role of ET‐1 on melanocyte proliferation. Our results of time‐lapse microphotography revealed a stimulatory effect of narrow‐band UVB irradiation on melanocyte migration. Focal adhesion kinase (FAK) plays a pivotal role in cell migration. Phosphorylated FAK (p125 FAK) expression on melanocyte was enhanced by narrow‐band UVB irradiation. In this study, narrow‐band UVB irradiation stimulated a significant increase in matrix metalloproteinase‐2 (MMP‐2) activity in melanocyte supernatants. Narrow‐band UVB‐irradiation‐induced migration of melanocytes was significantly annihilated by the addition of p125 FAK inhibitor (herbimycin‐A) or MMP‐2 inhibitor (GM6001). These results suggest that p125 FAK and MMP‐2 activity play important roles in narrow‐band UVB‐induced migration of melanocytes. Our results provide a theoretical basis for the effectiveness of narrow‐band UVB irradiation in treating vitiligo.

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Crossref · 10.1111/j.0906-6705.2004.00221.x

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Authors
Wu, C., Yu, C., Wu, C., Lan, C. E., & Yu, H.
Affiliations
  • Kaohsiung Medical University (TW)
  • National Taiwan University (TW)
Corresponding author
Yu, Hsin‐Su
ORCID
Venue
Experimental Dermatology
Publisher
Wiley
Published
2004-11-18
Volume
13
Issue
12
Pages
755-763
ISSN
  • 0906-6705 (ISSN-L)
  • 1600-0625
DOI
10.1111/j.0906-6705.2004.00221.x
OpenAlex
W2072562941
Cited by
121
References
1
Citations by year
2026: 4 · 2025: 3 · 2024: 5 · 2022: 4 · 2021: 6 · 2020: 7 · 2019: 6 · 2018: 12 · 2017: 13 · 2016: 11 · 2015: 7 · 2014: 10 · 2013: 5 · 2012: 3
FWCI
1.53
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Experimental Dermatology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Council
  • National Health Research Institutes
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Melanoma and MAPK Pathways
  • Health Sciences › Medicine › Dermatology › Dermatologic Treatments and Research
Keywords
narrowband UVB, vitiligo, melanocyte proliferation, melanocyte migration, basic fibroblast growth factor, endothelin-1, endothelin B receptor, focal adhesion kinase, MMP-2, repigmentation, herbimycin A, GM6001
MeSH terms
Cell Movement, Cell Survival, Cells, Cultured, Dipeptides, DNA, Enzyme-Linked Immunosorbent Assay, Humans, Melanocytes, Microscopy, Fluorescence, Oligopeptides, Phosphorylation, Piperidines, Protein-Tyrosine Kinases, Time Factors, Ultraviolet Rays, Keratinocytes, Fibroblast Growth Factor 2, Endothelin-1, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Cell Proliferation, Focal Adhesion Protein-Tyrosine Kinases, Focal Adhesion Kinase 1

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