CoChat
Sign Up
Cited by 3Angiogenesis and VEGF in CancerMyasthenia Gravis and ThymomaVascular Tumors and Angiosarcomas

Neoplastic transformation and angiogenesis in the thymus of transgenic mice expressing SV40 T and t antigen under an l‐pyruvate kinase promoter (SV12 mice)

Open access
Nabarra, Bernadette, Pontoux, Christiane, Godard, Cecile, Osborne‐Pellegrin, Mary, Ezine, Sophie·2005·International Journal of Experimental Pathology
DOI 10.1111/j.0959-9673.2005.00451.xPMC PMC2517450
Open this paper in CoChat — free account

Abstract

Summary: Using several techniques, we have assessed morphological characteristics of a malignant thymic tumour in SV12 transgenic (Tg) mice expressing SV40 T and t antigens under control of an L‐PK promoter. We describe the development of a carcinoma originating from thymic hyperplasia and followed by the formation of a benign tumour composed chiefly of medullary epithelial cells expressing the transgene and of lymphocytes, a pathology very rarely reported in mice. Our study of the SV12 Tg mice represents the first description of a model of a pure malignant thymic tumour associated with extensive angiogenesis maintained in numerous descendants. The formation of a large tumoral neovascular network, observed here, has never been described in human and/or experimental thymic tumours. Tumoral transformation and angiogenesis are demonstrated by immunolabelling with antibodies against various cytokeratins (CKs) of different molecular weights, vascular endothelial cell markers and VEGF/receptor‐2 (Flk‐1) present on the neovascular endothelial cells. Different points raised by the originality of this model are discussed. These include the medullary nature of the cells expressing the SV40 transgene and their relationship with the tumoral development. The subset of different molecular weight CK components and their modifications are also considered, as well as the presence of type IV epithelial cells, progenitors of medullary epithelial cells. Finally, the cell signals involved in angiogenesis and the possible action of an angiogenic factor, probably secreted by the tumoral cells themselves, are discussed.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1111/j.0959-9673.2005.00451.x

Cites

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The bladder cancer cell line T24 was established in 1972 and has contributed to several cell line cross-contamination.

    “The bladder cancer cell line T24 was established in 1972 and has contributed to several cell line cross-contamination [20, 21].”

    From Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines (2017) · cites this paper as [21]

    The Abstract and Materials and methods describe SV12 transgenic mice and thymic tumours, not the T24 bladder cancer cell line. Searches for T24, its establishment in 1972, and cell-line cross-contamination found no passage addressing either part of the claim. (LLM, conf=0.75)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

No references could be resolved yet.

Research works printed but not linked: 96

  1. Adams JM, Cory S. Transgenic models of tumor development. Science. 1991;254:1161–1167. doi: 10.1126/science.1957168. [DOI] [PubMed] [Google Scholar] · DOI
  2. Bennett AR, Farley A, Blair NF, Gordon J, Sharp L, Blackburn CC. Identification and characterization of thymic epithelial progenitor cells. Immunity. 2002;16:803–814. doi: 10.1016/s1074-7613(02)00321-7. [DOI] [PubMed] [Google Scholar] · DOI
  3. Berse B, Brown LF, Van de Water L, Dvorak HF, Senger DR. Vascular permeability factor (vascular endothelial growth factor) gene is expressed differentially in normal tissues, macrophages and tumors. MolBiolCell. 1992;3:211–220. doi: 10.1091/mbc.3.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  4. Bicknell R, Harris AL. Novel growth regulatory factors and tumor angiogenesis. EurJCancer. 1991;27:781–785. doi: 10.1016/0277-5379(91)90189-k. [DOI] [PubMed] [Google Scholar] · DOI
  5. Blackburn CC, Augustine CL, Li R, et al. The nu gene acts cell-autonomously and is required for differentiation of thymic epithelial progenitors. ProcNatlAcadSciUSA. 1996;93:5742–5746. doi: 10.1073/pnas.93.12.5742. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  6. Botteri FM, Van der Putten H, Wong DF, Sauvage CA, Evans RM. Unexpected thymic hyperplasia in transgenic mice harboring a neuronal promoter fused with simian virus 40 large T antigen. MolCell Biol. 1987;7:3178–3184. doi: 10.1128/mcb.7.9.3178-3184.1987. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  7. Bouck N. Angiogenesis: a mechanism by which oncogenes and tumor supressor genes regulate tumorigenesis. Cancer TreatRes. 1992;63:359–371. doi: 10.1007/978-1-4615-3088-6_19. [DOI] [PubMed] [Google Scholar] · DOI
  8. Boyd RL, Tucek CL, Godfrey DI, et al. The thymic microenvironment. ImmunolToday. 1993;14:445–459. doi: 10.1016/0167-5699(93)90248-J. [DOI] [PubMed] [Google Scholar] · DOI
  9. Breier G, Albrecht U, Sterrer S, Risau W. Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation. Development. 1992;114:521–532. doi: 10.1242/dev.114.2.521. [DOI] [PubMed] [Google Scholar] · DOI
  10. Brekelmans P, Van Ewijk W. Phenotypic characterization of murine thymic micro-environment. Semin Immunol. 1990;2:13–24. [PubMed] [Google Scholar]
  11. Brinster RL, Chen HY, Messing A, Van Dyke T, Levine AJ, Palmiter RD. Transgenic mice harboring SV40 T-antigen genes develop characteristic brain tumors. Cell. 1984;37:367–379. doi: 10.1016/0092-8674(84)90367-2. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  12. Burri P, Djonov V. Intussuceptive angiogenesis, the alternative to capillary sprouting. MolAspects Med. 2002;23:S1–S27. doi: 10.1016/s0098-2997(02)00096-1. [DOI] [PubMed] [Google Scholar] · DOI
  13. Carmeliet P. Mechanisms of angiogenesis and arteriogenesis. NatMed. 2000;6:389–395. doi: 10.1038/74651. [DOI] [PubMed] [Google Scholar] · DOI
  14. Cockerill GW, Gamble JR, Vadas MA. Angiogenesis models and modulators. Int. Rev. Cytol. 1995;159:113–160. doi: 10.1016/s0074-7696(08)62106-3. [DOI] [PubMed] [Google Scholar] · DOI
  15. Connolly DT, Heuvelman DM, Nelson R, et al. Tumor vascular permeability factor stimulates endothelial cell growth and angiogenesis. J. Clin. Invest. 1989;84:1470–1478. doi: 10.1172/JCI114322. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  16. Cooper D, Schermer A, Sun TT. Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations. LabInvest. 1985;52:243–256. [PubMed] [Google Scholar]
  17. De Lisser HM, Christofidou-Solomidou M, Strieter RM, et al. Involvement of endothelial PECAM/CD31 in angiogenesis. Am. J. Pathol. 1997;151:671–677. [PMC free article] [PubMed] [Google Scholar]
  18. Debus E, Moll R, Francke WW, Weber K, Osborn M. Immunohistochemical distinction of human carcinomas by cytokeratin typing with monoclonal antibodies. Am. J. Pathol. 1984;114:121–130. [PMC free article] [PubMed] [Google Scholar]
  19. Dvorak HF, Brown LF, Detmar M, Dvorak AM. Vascular permeability factor/vascular endothelial growth factor, microvascular, hyperpermeability and angiogenesis. Am. J. Pathol. 1995;146:1029–1039. [PMC free article] [PubMed] [Google Scholar]
  20. Efrat S, Baekkeskov S, Lane D, Hanahan D. Coordinate expression of the endogenous p53 gene in beta cells of transgenic mice expressing hybrid insulin-SV40 T antigen genes. EMBO J. 1987;6:2699–2704. doi: 10.1002/j.1460-2075.1987.tb02562.x. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  21. Espinoza CG, Azar HA. Immunohistochemical localization of keratin-type proteins in epithelial neoplasms. Am. J. ClinPathol. 1982;78:500–507. doi: 10.1093/ajcp/78.4.500. [DOI] [PubMed] [Google Scholar] · DOI
  22. Ferrara N. The role of vascular endothelial growth factor in pathological angiogenesis. Breast Cancer ResTreat. 1995;36:127–137. doi: 10.1007/BF00666035. [DOI] [PubMed] [Google Scholar] · DOI
  23. Fina L, Molgaard HV, Robertson D, et al. Expression of the CD34 gene in vascular endothelial cells. Blood. 1990;75:2417–2426. [PubMed] [Google Scholar]
  24. Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other diseases. NatMed. 1995;1:27–31. doi: 10.1038/nm0195-27. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  25. Folkman J, Klagsbrun M. Angiogenic factors. Science. 1987;235:442–447. doi: 10.1126/science.2432664. [DOI] [PubMed] [Google Scholar] · DOI
  26. Folkman J, Watson K, Ingber D, Hanahan D. Induction of angiogenesis during the transition from hyperplasia to neoplasia. Nature. 1989;359:58–61. doi: 10.1038/339058a0. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  27. Form DM, Pratt BM, Madri JA. Endothelial cell proliferation during angiogenesis. In vitro modulation of basement membrane components. LabInvest. 1986;55:521–530. [PubMed] [Google Scholar]
  28. Franke WW, Schiller DL, Moll R, et al. Diversity of cytokeratins: differentiation specific expression of cytokeratin polypeptides in epithelial cells and tissues. J. Mol. Biol. 1981;153:933–959. doi: 10.1016/0022-2836(81)90460-5. [DOI] [PubMed] [Google Scholar] · DOI
  29. Frith CH, Ward JM, Chandra M. The morphology, immunochemistry and incidence of hematopoietic neoplasm in mice and rats. ToxicolPathol. 1993;21:206–218. doi: 10.1177/019262339302100213. [DOI] [PubMed] [Google Scholar] · DOI
  30. Fukai I, Masaoka A, Hashimoto T, Yamakawa Y, Mizuno T, Tanamura O. Cytokeratins in normal thymus and thymic epithelial tumors. Cancer. 1993;71:99–105. doi: 10.1002/1097-0142(19930101)71:1<99::aid-cncr2820710116>3.0.co;2-6. [DOI] [PubMed] [Google Scholar] · DOI
  31. Galy AH, de Waal Malefyt R, Barcena A, Mohan-Peterson S, Spits H. Untransfected and SV40-transfected fetal and post-natal human thymic stroma cells. Thymus. 1993;22:13–33. [PubMed] [Google Scholar]
  32. Gill J, Malin M, Hollander GA, Boyd R. Generation of a complete thymic micro-environment by MTS24+ thymic epithelial cells. Nat Immunol. 2002;3:635–642. doi: 10.1038/ni812. [DOI] [PubMed] [Google Scholar] · DOI
  33. Hanahan D. Oncogenes and Growth Control. Berlin: Springer Verlag; 1986. Oncogenesis in transgenic mice; pp. 349–363. [Google Scholar]
  34. Hanahan D. Dissecting multistep tumorigenesis in transgenic mice. Annu. Rev. Genet. 1988;22:479–519. doi: 10.1146/annurev.ge.22.120188.002403. [DOI] [PubMed] [Google Scholar] · DOI
  35. Hanahan D. Signaling vascular morphogenesis and maintenance. Science. 1997;277:48–50. doi: 10.1126/science.277.5322.48. [DOI] [PubMed] [Google Scholar] · DOI
  36. Hanahan D, Folkman J. Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell. 1996;86:335–364. doi: 10.1016/s0092-8674(00)80108-7. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  37. Ho FC, Fu KH, Lam SY, Chiu SW, Chan AC, Muller-Hermelink HK. Evaluation of a histogenetic classification for thymic epithelial tumors. Histopathology. 1994;25:21–29. doi: 10.1111/j.1365-2559.1994.tb00594.x. [DOI] [PubMed] [Google Scholar] · DOI
  38. Hoot GP, Kettman JR. Primary polyoma virus-induced murine thymic epithelial tumors. Am. J. Pathol. 1989;135:679–695. [PMC free article] [PubMed] [Google Scholar]
  39. Hornis TS, Steinberg ML, Defendi V, Sun TT. Simple epithelial nature of simian virus-40-transformed human epidermal keratinocytes. Cancer Res. 1984;44:5797–5804. [PubMed] [Google Scholar]
  40. Ingber DE, Folkman J. How does extracellular matrix control capillary morphogenesis. Cell. 1989;58:803–805. doi: 10.1016/0092-8674(89)90928-8. [DOI] [PubMed] [Google Scholar] · DOI
  41. Jakeman LB, Winer J, Bennett GL, Altar CA, Ferrara N. Binding sites for vascular endothelial growth factor are localized on endothelial cells in adult rat tissue. J. Clin. Invest. 1992;89:244–253. doi: 10.1172/JCI115568. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  42. Kirchner T, Muller-Hermelink HK. New approaches to the diagnosis of thymic epithelial tumors. Prog. Surg. Pathol. 1989;10:167–189. [Google Scholar]
  43. Klagsbrun M, D'Amore PA. Regulation of angiogenesis. Annu. Rev. Physiol. 1991;53:217–239. doi: 10.1146/annurev.ph.53.030191.001245. [DOI] [PubMed] [Google Scholar] · DOI
  44. Klagsbrun M, Soker S. VEGF/VPF: the angiogenesis factor found? Current Biol. 1993;10:699–702. doi: 10.1016/0960-9822(93)90073-w. [DOI] [PubMed] [Google Scholar] · DOI
  45. Klug DB, Carter C, Crouch D, Roop C, Gimenez-Conti CJ, Richie ER. Interdependence of cortical thymic epithelial cell differentiation and T-lineage commitment. ProcNatlAcadSciUSA. 1998;95:11822–11824. doi: 10.1073/pnas.95.20.11822. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  46. Klug DB, Carter C, Gimenez-Conti IB, Richie ER. Cutting edge: Thymocyte-independent and thymocyte-dependent phases of epithelial patterning in the fetal thymus. JImmunol. 2002;169:2842–2845. doi: 10.4049/jimmunol.169.6.2842. [DOI] [PubMed] [Google Scholar] · DOI
  47. Kornstein M, Deblois G. Tumors of the thymic epithelial cells. In: Kornstein) M, editor. Pathology of the Thymus and Mediastinum. Philadelphia: W. Saunders.; 1995. pp. 67–113. [Google Scholar]
  48. Kuo TT, Chan JK. Thymic carcinoma arising in thymoma is associated with alterations in immunohistochemical profile. Am. J. SurgPathol. 1998;22:1474–1481. doi: 10.1097/00000478-199812000-00004. [DOI] [PubMed] [Google Scholar] · DOI
  49. Lane DP, Crawford LV. T antigen is bound to a host protein in SV40-transformed cells. Nature. 1979;278:261–263. doi: 10.1038/278261a0. [DOI] [PubMed] [Google Scholar] · DOI
  50. Ledinko N, Costantino RL. Modulation of p53 gene expression of cytokeratin 18 in retinoid-mediated invasion suppressed lung carcinoma cells. Anticancer Res. 1990;10:1335–1339. [PubMed] [Google Scholar]
  51. Lee SS, Park WY, Chi JG, et al. Thymic epithelial tumor progression in an SV40 T transgenic mouse model. Virchows Arch. 1998;432:33–42. doi: 10.1007/s004280050131. [DOI] [PubMed] [Google Scholar] · DOI
  52. Leung DW, Cachianes G, Kuang WJ, Goeddel DV, Ferrara N. Vascular endothelial growth factor is a secreted angiogenic mitogen. Science. 1989;246:1306–1309. doi: 10.1126/science.2479986. [DOI] [PubMed] [Google Scholar] · DOI
  53. Levine AJ, Perry ME, Chang A, et al. The role of the p53 tumor-suppressor gene in tumorigenesis. BrJCancer. 1994;69:409–416. doi: 10.1038/bjc.1994.76. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  54. Longacre T, Rouse R. CD31: a new marker for vascular neoplasia. Adv. Anat. Pathol. 1994;1:16–20. [Google Scholar]
  55. Loning T, Viac J, Caselitz J, Thivolet J, Otto HF, Seifert G. Comparative investigation of keratin filaments in normal tissues and tumours of skin, oral mucosa, salivary glands and thymus. Pathol. Res. Pract. 1982;175:256–265. doi: 10.1016/S0344-0338(82)80112-X. [DOI] [PubMed] [Google Scholar] · DOI
  56. Ludlow JW. Interaction between SV40 large-tumor antigen and the growth suppressor proteins pRb and p53. FASEB J. 1993;5:866–871. doi: 10.1096/fasebj.7.10.8344486. [DOI] [PubMed] [Google Scholar] · DOI
  57. Matsuyama M, Suzuki H, Yamada S, Ito M, Nagayo T. Ultrastructure of spontaneous and urethan-induced thymomas in Buffalo rats. Cancer Res. 1975;35:2771–2779. [PubMed] [Google Scholar]
  58. McCarthy SA, Symonds HS, Van Dyke T. Regulation of apoptosis in transgenic mice by simian virus 40 T antigen-mediated inactivation of p53. ProcNatlAcadSciUSA. 1994;91:3979–3983. doi: 10.1073/pnas.91.9.3979. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  59. Messing A, Pinkert CA, Palmiter RD, Brinster RL. Developmental study of SV40 large T antigen expression in transgenic mice with choroid plexus neoplasia. Oncogene Res. 1988;3:87–97. [PubMed] [Google Scholar]
  60. Millauer B, Wizigmann-Voos S, Schnurch H, et al. High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis. Cell. 1993;72:835–846. doi: 10.1016/0092-8674(93)90573-9. [DOI] [PubMed] [Google Scholar] · DOI
  61. Moll J, Eibel H, Botteri F, Sansig G, Regnier C, van der Putten H. Transgenes encoding mutant simian virus 40 large T antigens unmask phenotypic and functional constraints in thymic epithelial cells. Oncogene. 1992;7:2175–2187. [PubMed] [Google Scholar]
  62. Moll R, Krepler R, Franke WW. Complex cytokeratin polypeptide patterns observed in certain human carcinomas. Differentiation. 1983;23:256–269. doi: 10.1111/j.1432-0436.1982.tb01291.x. [DOI] [PubMed] [Google Scholar] · DOI
  63. Moroco JR, Solt DB, Polverini PJ. Sequential loss of suppressor genes for three specific functions during in vivo carcinogenesis. LabInvest. 1990;63:298–306. [PubMed] [Google Scholar]
  64. Muller-Hermelink H, Marx A, Kirchner T. Advances in the diagnosis and classification of thymic epithelial tumors. Rec. Adv. Histopathol. 1994;16:49–72. [Google Scholar]
  65. Murray AB, Schaffer E, Nussel M, LuZ A. Incidence, morphology and ultrastructure of spontaneous thymoma, the most common neoplasm in W/Nhg rats. JNatlCancer Inst. 1985;75:369–379. [PubMed] [Google Scholar]
  66. Nabarra B, Andrianarison I. Ultrastructural studies of thymic reticulum. I. Epithelial components. Thymus. 1987;9:95–121. [PubMed] [Google Scholar]
  67. Nabarra B, Mulotte M, Casanova M, Godard C, London J. Ultrastructural study of the FVB/N mouse thymus: presence of an immature epithelial cell in the medulla and premature involution. Dev. Comp. Immunol. 2001;25:231–243. doi: 10.1016/s0145-305x(00)00054-9. (Erratum in Dev. Comp. Immunol. 25, 539–543). [DOI] [PubMed] [Google Scholar] · DOI
  68. Nabarra B, Martinon C, Godard C, et al. Early steps of a thymic tumor in SV40 transgenic mice: hyperplasia of medullary epithelial cells and increased mature thymocyte number disturb thymic export. DevImmunol. 2002;9:223–231. doi: 10.1080/10446670310001593532. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  69. Naylor DC, Krinke GJ, Ruefenacht HJ. Primary tumors of the thymus in the rat. J. Comp. Pathol. 1988;99:187–203. doi: 10.1016/0021-9975(88)90071-0. [DOI] [PubMed] [Google Scholar] · DOI
  70. Neufeld G, Tessler S, Gitay-Goren H, Cohen T, Levi BZ. Vascular endothelial growth factor and its receptors. Prog. Growth Fact. Res. 1994;5:89–97. doi: 10.1016/0955-2235(94)90019-1. [DOI] [PubMed] [Google Scholar] · DOI
  71. Palmiter R, Chen HY, Messing A, Brinster RL. SV40 enhancer and large T antigen are instrumental in development of choroid plexus tumors in transgenic mice. Nature. 1985;316:457–460. doi: 10.1038/316457a0. [DOI] [PubMed] [Google Scholar] · DOI
  72. Park WY, Kim JI, Shim EH, et al. Development of thymic carcinoma in transgenic mice expressing SV40 T antigen. Cancer Lett. 1996;107:293–300. doi: 10.1016/0304-3835(96)04413-8. [DOI] [PubMed] [Google Scholar] · DOI
  73. Plate KH, Breir G, Weich HA, Risau W. Vascular endothelial growth is a potential tumor angiogenesis factor in vivo. Nature. 1992;359:845–848. doi: 10.1038/359845a0. [DOI] [PubMed] [Google Scholar] · DOI
  74. Quinn TP, Peters KG, De Vries C, Ferrara N, Williams LT. Fetal liver kinase-1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium. ProcNatlAcadSciUSA. 1993;90:7533–7537. doi: 10.1073/pnas.90.16.7533. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  75. Rak J, Filmus J, Finkenzeller G, Grugel S, Marme D, Kerbel RS. Oncogenes as inductors of tumor angiogenesis. Cancer Metastasis Rev. 1995;14:263–277. doi: 10.1007/BF00690598. [DOI] [PubMed] [Google Scholar] · DOI
  76. Ramaekers F, Huysmans A, Moesker O, Schaart O, Herman C, Vooijs P. Cytokeratin expression during neoplastic progression of human transitional cell carcinomas as detetected by monoclonal and polyclonal antibodies. LabInvest. 1985;52:31–38. [PubMed] [Google Scholar]
  77. Reynolds RK, Hoekzema GS, Vogel J, Hinrichs SH, Jay G. Multiple endocrine neoplasia induced by the promiscuous expression of a viral oncogen. ProcNatlAcadSciUSA. 1988;85:3135–3139. doi: 10.1073/pnas.85.9.3135. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  78. Risau W. Vasculogenesis, angiogenesis and endothelial cell differentiation during embryonic development. In: Feinberg R, Sherer G, Auerbach R, editors. The Development of Vascular System. Basel: Karger; 1991. pp. 58–68. [Google Scholar]
  79. Rosai J, Levine G. Atlas of Tumor Pathology. Washington, DC: : Armed Forces Institute of Pathology.; 1976. Tumors of the thymus. [Google Scholar]
  80. Saenz-Robles MT, Symonds H, Chen J, Van Dyke T. Induction versus progression of brain tumor development: differential functions for pRb- and p53-targeting domains of simian virus 40 T antigen. MolCell Biol. 1994;14:2686–2698. doi: 10.1128/mcb.14.4.2686-2698.1994. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  81. Savino W, Dardenne M. Immunohistochemical studies on a human thymic epithelial cell subset defined by the anti-cytokeratin 18 monoclonal antibody. Cell Tissue Res. 1988;254:225–231. doi: 10.1007/BF00220038. [DOI] [PubMed] [Google Scholar] · DOI
  82. Schlingemann RO, Rietveld FJ, de Waal RM, et al. Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma. LabInvest. 1990;62:690–696. [PubMed] [Google Scholar]
  83. Schor AM, Schor SL. Tumor angiogenesis. JPathol. 1983;141:385–413. doi: 10.1002/path.1711410315. [DOI] [PubMed] [Google Scholar] · DOI
  84. Senger DR, Van de Water L, Brown LF. Vascular permeability factor (VPF/VEGF) in tumor biology. Cancer Metastasis Rev. 1993;12:303–324. doi: 10.1007/BF00665960. [DOI] [PubMed] [Google Scholar] · DOI
  85. Sepulveda AR, Finegold MJ, Smith B, et al. Development of a transgenic mouse system for the analysis of stages in liver carcinoma genesis using tissue-specific expression of SV40 large T-antigen controlled by regulatory elements of the human alpha-1-antitrypsin gene. Cancer Res. 1989;49:6108–6117. [PubMed] [Google Scholar]
  86. Shalaby F, Ho J, Standford WL, et al. A requirement for Flk-1 in primitive and definitive hematopoiesis and vasculogenesis. Cell. 1997;89:981–990. doi: 10.1016/s0092-8674(00)80283-4. [DOI] [PubMed] [Google Scholar] · DOI
  87. Small JA, Blair DG, Showalter SD, Scangos GA. Analysis of a transgenic mouse containing SV40 and v-myc sequence. Mol. Cell. Biol. 1985;5:642–648. doi: 10.1128/mcb.5.4.642-648.1985. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  88. Suster S, Moran CA. Thymic carcinoma: spectrum of differentiation and histologic types. Pathology. 1998;30:111–122. doi: 10.1080/00313029800169056. [DOI] [PubMed] [Google Scholar] · DOI
  89. Suster S, Moran CA. Primary thymic epithelial neoplasms: spectrum of differentiation and histological features. Semin. Diagn. Pathol. 1999;16:2–17. [PubMed] [Google Scholar]
  90. Tamano S, Hagiwara A, Shibata MA, Kurata Y, Fukushima S, Ito N. Spontaneous tumors in aging (C57BL/6N × C3H/HeN) F1 (B6C3F1) mice. ToxicolPathol. 1988;16:321–326. doi: 10.1177/019262338801600302. [DOI] [PubMed] [Google Scholar] · DOI
  91. Teitz T, Chang JC, Kan YW, Yen TS. Thymic epithelial neoplasm in transgenic mice expressing SV40 T antigen under the control of an erythroid-specific enhancer. JPathol. 1995;177:309–315. doi: 10.1002/path.1711770314. [DOI] [PubMed] [Google Scholar] · DOI
  92. Thomas P, Said JW, Nash G, Banks-Schlegel S. Profiles of keratin proteins in basal and squamous cell carcinomas in the skin. An immunohistochemical study. LabInvest. 1984;50:36–41. [PubMed] [Google Scholar]
  93. Truong LD, Mody DR, Cagle PT, Jackson-York GL, Schwartz MR, Wheeler TM. Thymic carcinoma. A clinicopathologic study of 13 cases. Am. J. SurgPathol. 1990;14:151–166. doi: 10.1097/00000478-199002000-00007. [DOI] [PubMed] [Google Scholar] · DOI
  94. Tseng SC, Jarvinen MJ, Nelson WG, Huang JW, Woodcock-Mitchell J, Sun TT. Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies. Cell. 1982;30:361–372. doi: 10.1016/0092-8674(82)90234-3. [DOI] [PubMed] [Google Scholar] · DOI
  95. Van Dyke TA, Finlay C, Miller D, Marks J, Lozano G, Levine AJ. Relationship between simian virus 40 large tumor antigen expression and tumor formation in transgenic mice. JVirol. 1987;61:2029–2032. doi: 10.1128/jvi.61.6.2029-2032.1987. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  96. Ward JM, Rehm S. Applications of immunohistochemistry in rodent tumor pathology. ExpPathol. 1990;40:301–312. doi: 10.1016/s0232-1513(11)80317-8. [DOI] [PubMed] [Google Scholar] · DOI

Cited by

Sorted by citations

Authors
Nabarra, B., Pontoux, C., Godard, C., Osborne‐Pellegrin, M., & Ezine, S.
Affiliations
Inserm (FR)
Corresponding author
Nabarra, Bernadette
Venue
International Journal of Experimental Pathology
Publisher
Wiley
Published
2005-11-24
Volume
86
Issue
6
Pages
397-413
ISSN
  • 0959-9673 (ISSN-L)
  • 1365-2613
DOI
10.1111/j.0959-9673.2005.00451.x
PMC
PMC2517450
OpenAlex
W1823981504
Cited by
3
References
0
Citations by year
2017: 1 · 2014: 1
FWCI
0.00
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • International Journal of Experimental Pathology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
  • Health Sciences › Medicine › Neurology › Myasthenia Gravis and Thymoma
  • Health Sciences › Medicine › Oncology › Vascular Tumors and Angiosarcomas
Keywords
thymic carcinoma, SV40 T antigen, transgenic mice, tumor angiogenesis, thymic hyperplasia, cytokeratin, tumor models
MeSH terms
Animals, Antigens, Polyomavirus Transforming, Antigens, Viral, Tumor, Cell Transformation, Neoplastic, Female, Immunohistochemistry, Male, Mice, Inbred CBA, Mice, Transgenic, Neovascularization, Pathologic, Promoter Regions, Genetic, Pyruvate Kinase, Thymus Gland, Thymus Neoplasms, Biomarkers, Tumor, Microscopy, Immunoelectron, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review