The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Preparation of Suspensions of Washed Platelets from Humans
Abstract
Summary: Methods have been developed for the preparation of suspensions of washed platelets from humans. Heparin is used in the washing fluids to prevent: thrombin generation and apyrase is used to prevent adenine nucleotide accumulation. Platelets suspended in Eagle's tissue culture medium containing albumin were more responsive to ADP than platelets in Tyrode's‐albumin solution. Addition of fibrinogen is required for maximum sensitivity to ADP‐induced aggregation. These platelets can be stored for 4 hr or more at 37°C in the presence of apyrase and maintain their ability to aggregate upon the addition of low concentrations of ADP. Without apyrase the platelets gradually become insensitive to ADP upon storage at 37°C; this is presumably caused by the accumulation of ADP in the suspending fluid because sensitivity can be partially restored by the addition of apyrase and further incubation.
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Citations
Cites
The aggregation of blood platelets
Born, G. V. R., & Cross, M. J. (1963) · The Journal of Physiology · doi:10.1113/jphysiol.1963.sp007185 · Cited by 2,111
Platelet Sequestration in Man. I. Methods*
Aster, R. H., & Jandl, J. H. (1964) · Journal of Clinical Investigation · doi:10.1172/jci104970 · Cited by 816
Adenine nucleotide metabolism of blood platelets
Rozenberg, M. C., & Holmsen, H. (1968) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(68)90082-8 · Cited by 69
Plasma Co-factors in Adenosine Diphosphate-induced Aggregation of Human Platelets
Deykin, D., Pritzker, C. R., & Scolnick, E. M. (1965) · Nature · doi:10.1038/208296b0 · Cited by 65
Cited by
Weksler, B. B., Ley, C. W., & Jaffe, E. A. (1978) · Journal of Clinical Investigation · doi:10.1172/jci109220 · Cited by 716
Human platelets possess an inducible and saturable receptor specific for fibrinogen.
Marguerie, G. A., Plow, E. F., & Edgington, T. S. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50603-7 · Cited by 706
Platelet hypersensitivity induced by cholesterol incorporation.
Shattil, S. J., Anaya-Galindo, R., Bennett, J., Colman, R. W., & Cooper, R. A. (1975) · Journal of Clinical Investigation · doi:10.1172/jci107971 · Cited by 461
Huang, T. F., Holt, J. C., Lukasiewicz, H., & Niewiarowski, S. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47710-1 · Cited by 324
Fibrinogen and ADP-induced platelet aggregation
Mustard, J., Packham, M., Kinlough-Rathbone, R., Perry, D., & Regoeczi, E. (1978) · Blood · doi:10.1182/blood.v52.2.453.453 · Cited by 303
Self-citation
Metadata
- Authors
- Mustard, J. F., Perry, D. W., Ardlie, N. G., & Packham, M. A.
- Venue
- British Journal of Haematology
- Publisher
- Wiley
- Published
- 1972-02
- Volume
- 22
- Issue
- 2
- Pages
- 193-204
- ISSN
- 0007-1048 (ISSN-L)
- 1365-2141
- OpenAlex
- W2035423234
- Cited by
- 831
- References
- 4
- FWCI
- 21.10
- Type
- journal article
- Language
- en
- Open access
- closed
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