The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Cell cycle dependent expression and stability of the nuclear protein detected by Ki‐67 antibody in HL‐60 cells
Abstract
Abstract. The expression and stability of the proliferation‐associated nuclear antigen detected by Ki‐67 antibody have been investigated in human promyelocytic leukaemic HL‐60 cells in relation to their progression through the cell cycle. Expression of this antigen was minimal in late G 1 and early S phase cells. The antigen accumulated in the cells predominantly during S phase, and its rate of increase per cell accelerated during the second half of this phase. The accumulation of Ki‐67 antigen during S exceeded the increase in DNA content, and thus the Ki‐67/DNA ratio rose 80% from late G 1 to G 2+ M. This antigen rapidly disappeared from post‐mitotic cells. The half‐life of this protein estimated in post‐mitotic cells during stathmokinesis induced by vinblastine appeared to be shorter than 1 h. This rapid turnover should be compared with the relatively long (6–8 h) duration of G 1 of the studied cells. In cells in which de novo protein synthesis was inhibited by 0.1 μg/ml cycloheximide, the half‐life of the Ki‐67 antigen was also found to be about 1 h regardless of the cell position in the cell cycle. Thus, the data suggest that variations in the level of this protein during the cell cycle are a consequence of its different synthesis rate rather than phase‐specific changes in the rate of its degradation. Because the late G 1 and very early S phase cells express the antigen at levels only slightly above background, it is possible that, when using Ki‐67 antibody as a marker of the cell growth fraction, some late G 1 cells can be erroneously classified as non‐cycling cells.
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The cell cycle associated change of the Ki‐67 reactive nuclear antigen expression
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Ki67 in breast cancer: prognostic and predictive potential
Yerushalmi, R., Woods, R., Ravdin, P. M., Hayes, M. M., & Gelmon, K. A. (2010) · The Lancet Oncology · doi:10.1016/s1470-2045(09)70262-1 · Cited by 1,207
In vivo Switching of Human Melanoma Cells between Proliferative and Invasive States
Hoek, K. S., Eichhoff, O. M., Schlegel, N. C., Döbbeling, U., Kobert, N., Schaerer, L., Hemmi, S., & Dummer, R. (2008) · Cancer Research · doi:10.1158/0008-5472.can-07-2491 · Cited by 743
Pampusch, M. S., Haran, K. P., Hart, G. T., Rakasz, E. G., Rendahl, A. K., Berger, E. A., Connick, E., & Skinner, P. J. (2020) · Molecular Therapy - Methods & Clinical Development · doi:10.1016/j.omtm.2019.09.007 · Cited by 41
Strzepa, A., Gurski, C. J., Dittel, L. J., Szczepanik, M., Pritchard, K. A., & Dittel, B. N. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2020.608871 · Cited by 26
Hippocampal proliferation is increased in presymptomatic Parkinson’s disease and due to microglia
Doorn, K. J., Drukarch, B., van Dam, A.-M., & Lucassen, P. J. (2014) · Neural Plasticity · doi:10.1155/2014/959154 · Cited by 24
Metadata
- Authors
- Bruno, S., & Darzynkiewicz, Z.
- Venue
- Cell Proliferation
- Publisher
- Wiley
- Published
- 1992-01
- Volume
- 25
- Issue
- 1
- Pages
- 31-40
- ISSN
- 0960-7722 (ISSN-L)
- 1365-2184
- OpenAlex
- W2031821806
- Cited by
- 375
- References
- 26
- FWCI
- 6.56
- Type
- journal article
- Language
- en
- Open access
- closed
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- Bruno, S., & Darzynkiewicz, Z. (1992). Cell cycle dependent expression and stability of the nuclear protein detected by Ki‐67 antibody in HL‐60 cells. Cell Proliferation. https://doi.org/10.1111/j.1365-2184.1992.tb01435.x
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