A novel rat model for chemotherapy-induced alopecia: A rat model for chemotherapy-induced alopecia
Open accessAbstract
BACKGROUND: More than half of all people diagnosed with cancer receive chemotherapy, and approximately 65% of these develop chemotherapy-induced alopecia (CIA), a side-effect that can have considerable negative psychological repercussions. Currently, there are very few animal models available to study the mechanism and prevention of CIA. AIM: To develop a clinically relevant adult rat model for CIA. METHODS: We first tested whether neonatal pigmented Long-Evans (LE) rats developed alopecia in response to the chemotherapeutic agents etoposide and cyclophosphamide. We then determined whether the rats developed CIA as adults. In the latter experiment, rat dorsal hair was clipped during the early telogen stage to synchronize the hair cycle, and starting 15 days later, the rats were treated with etoposide for 3 days. RESULTS: Neonatal LE pups developed CIA in response to etoposide and cyclophosphamide, similar to other murine models for CIA. Clipping of the hair shaft during early telogen resulted in synchronized anagen induction and subsequent alopecia after etoposide treatment in the clipped areas only. Hair follicles in the clipped areas had the typical chemotherapy-induced follicular dystrophy (dystrophic catagen). When the hair in the pigmented alopecic areas regrew, it had normal pigmentation. CONCLUSIONS: A novel, pigmented adult rat model has been established for CIA. By hair-shaft clipping during early telogen, synchronized anagen entry was induced, which resulted in alopecia in response to chemotherapy. This is the first clinically relevant adult rat model for CIA, and will be a useful tool to test agents for the prevention and treatment of CIA.
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Clipping the dorsal hair of rats synchronizes the hair cycle after a certain period of time, so it may be difficult to compare results with animals that have not undergone this treatment.
“Clipping the dorsal hair of rats synchronizes the hair cycle after a certain period of time; therefore, it may be difficult to compare the results with those of animals that have not undergone this treatment48.”
From Morphological characteristics and notes of the skin in preclinical toxicity assessment (2023) · cites this paper as [48]
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“Mice, rats, and rabbits are the animal models that have been studied so far (Table 5) [178-182].”
BackgroundA Clinical and Biological Guide for Understanding Chemotherapy-Induced Alopecia and Its Prevention
Dunnill, C. J., Al-Tameemi, W., Collett, A., Haslam, I. S., & Georgopoulos, N. T. (2018) · The Oncologist · doi:10.1634/theoncologist.2017-0263 · Cited by 96 · see citing works
Morphological characteristics and notes of the skin in preclinical toxicity assessment
Moroki, T. (2023) · Journal of Toxicologic Pathology · doi:10.1293/tox.2022-0103 · Cited by 26 · see citing works
- Authors
- Wikramanayake, T. C., Amini, S., Simon, J., Mauro, L. M., Elgart, G., Schachner, L. A., & Jimenez, J. J.
- Affiliations
- University of Miami (US)
- Corresponding author
- Jimenez, J. J.
- Venue
- Clinical and Experimental Dermatology
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-03-12
- Volume
- 37
- Issue
- 3
- Pages
- 284-289
- ISSN
- 0307-6938 (ISSN-L)
- PMC
- PMC3305180
- OpenAlex
- W1482445622
- Cited by
- 30
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 4 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 4 · 2014: 4 · 2013: 2
- FWCI
- 3.68
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Clinical and Experimental Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR050487, K01AR050487)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Health Sciences › Medicine › Dermatology › Chemotherapy-related skin toxicity
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Keywords
- chemotherapy-induced alopecia, rat model, etoposide, cyclophosphamide, hair follicle, telogen, anagen induction, hair pigmentation, Long-Evans rats, chemotherapy side effects
- MeSH terms
- Alopecia, Animals, Antineoplastic Agents, Cyclophosphamide, Disease Models, Animal, Etoposide, Hair Follicle, Rats, Long-Evans, Rats
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