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Production and synthetic dyes decolourization capacity of a recombinant laccase from Pichia pastoris: Recombinant laccase in dye decolourization

Lu, L., Zhao, M., Liang, S.-C., Zhao, L.-Y., Li, D.-B., Zhang, B.-B.·2009·Journal of Applied Microbiology
DOI 10.1111/j.1365-2672.2009.04291.x
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Abstract

Aims To produce and purify a recombinant laccase from Pichia pastoris and to test its ability in decolourization of synthetic dyes. Methods and results A cDNA encoding for a laccase was isolated from Pycnoporus sanguineus and was expressed in P. pastoris strain SMD1168H under the control of the alcohol oxidase (AOX1) promoter. The laccase native signal peptide efficiently directed the secretion of the recombinant laccase in an active form. Factors influencing laccase expression, such as cultivation temperature, pH, copper concentration and methanol concentration, were investigated. The recombinant enzyme was purified to electrophoretic homogeneity, and was estimated to have a molecular mass of about 62.8 kDa. The purified enzyme showed a similar behaviour to the native laccase produced by P. sanguineus. Four different synthetic dyes including azo, anthraquinone, triphenylmethane and indigo dyes could be efficiently decolourized by the purified recombinant laccase without the addition of redox mediators. Conclusions Heterologous production of P. sanguineus laccase in P. pastoris was successfully achieved. The purified recombinant laccase could efficiently decolourize synthetic dyes in the absence of mediators. Significance and impact of the study This study is the first report on the synthetic dye decolourization by the recombinant P. sanguineus laccase. The decolourization capacity of this recombinant enzyme suggested that it could be a useful biocatalyst for the treatment of…

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  • Laccase examined for dye decolorising potential on several types of dyes showed better affinity towards the anthraquinone Reactive Blue 19, resulting in almost 90% decolorisation over 30 mins.

    “When laccase was examined for dye decolorising potential on several types of dyes, it was shown that there was better affinity towards the anthraquinone Reactive Blue 19, resulting in almost 90% decolorisation over 30 mins 153 .”

    From Degradation of anthraquinone dyes from effluents: A review focusing on enzymatic dye degradation with industrial potential (2020) · cites this paper as [153]

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Research works printed but not linked: 32

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Metadata

Authors
Lu, L., Zhao, M., Liang, S.-C., Zhao, L.-Y., Li, D.-B., & Zhang, B.-B.
Venue
Journal of Applied Microbiology
Publisher
Oxford University Press (OUP)
Published
2009-03-30
Volume
107
Issue
4
Pages
1149-1156
ISSN
1364-5072
DOI
10.1111/j.1365-2672.2009.04291.x
Cited by
72
References
0
Language
en
Licence
doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 2346 d)

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