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The ObgE/CgtA GTPase influences the stringent response to amino acid starvation in Escherichia coli
Open accessAbstract
Summary: The stringent response is important for bacterial survival under stressful conditions, such as amino acid starvation, and is characterized by the accumulation of ppGpp and pppGpp. ObgE (CgtA, YhbZ) is an essential conserved GTPase in Escherichia coli and several observations have implicated the protein in the control of the stringent response. However, consequences of the protein on specific responses to amino acid starvation have not been noted. We show that ObgE binds to ppGpp with biologically relevant affinity in vitro, implicating ppGpp as an in vivo ligand of ObgE. ObgE mutants increase the ratio of pppGpp to ppGpp within the cell during the stringent response. These changes are correlated with a delayed inhibition of DNA replication by the stringent response, delayed resumption of DNA replication after release, as well as a decreased survival after amino acid deprivation. With these data, we place ObgE as an active effector of the response to amino acid starvation in vivo. Our data correlate the pppGpp/ppGpp ratio with DNA replication control under bacterial starvation conditions, suggesting a possible role for the relative balance of these two nucleotides.
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“and competitive inhibition of GTP-binding proteins, like the ribosome assembly factor ObgE (Sato et al., 2005; Persky et al., 2009; Feng et al., 2014), the initiation factor IF2 (Diez et al., 2020) or elongation factor EF-G (Mitkevich et al., 2010; Steinchen et al., 2020).”
From The Dynamic SecYEG Translocon (2021) · cites this paper as Persky et al., 2009
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Hauryliuk, V., Atkinson, G. C., Murakami, K. S., Tenson, T., & Gerdes, K. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3448 · Cited by 810
The stringent response and physiological roles of (pp)pGpp in bacteria.
Irving, S. E., Choudhury, N. R., & Corrigan, R. M. (2021) · Nature Reviews Microbiology · doi:10.1038/s41579-020-00470-y · Cited by 413
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Metadata
- Authors
- Persky, N. S., Ferullo, D. J., Cooper, D. L., Moore, H. R., & Lovett, S. T.
- Affiliations
- Brandeis University (US)
- Corresponding author
- Lovett, Susan T.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2009-07
- Volume
- 73
- Issue
- 2
- Pages
- 253-266
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- PMC
- PMC2771346
- OpenAlex
- W2162380932
- Cited by
- 77
- References
- 55
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 7 · 2023: 1 · 2022: 3 · 2021: 6 · 2020: 6 · 2019: 6 · 2018: 5 · 2017: 3 · 2016: 8 · 2015: 5 · 2014: 6 · 2013: 5 · 2012: 4
- FWCI
- 2.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- U.S. Public Health Service (R01 G51753, R01 G79510, T32 G07596)
- National Institute of General Medical Sciences (T32GM007596, R01GM079510, R01GM051753)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- stringent response, GTPase, ppGpp, pppGpp, amino acid starvation, DNA replication inhibition, Escherichia coli, starvation survival
- MeSH terms
- Amino Acids, DNA Replication, DNA, Bacterial, Escherichia coli, Guanosine Tetraphosphate, Serine, Monomeric GTP-Binding Proteins, Escherichia coli Proteins
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- CoChat. (2026). Citation check: The ObgE/CgtA GTPase influences the stringent response to amino acid starvation in Escherichia coli. https://app.cochat.ai/papers/10.1111/j.1365-2958.2009.06767.x
- The paper
- Persky, N. S., Ferullo, D. J., Cooper, D. L., Moore, H. R., & Lovett, S. T. (2009). The ObgE/CgtA GTPase influences the stringent response to amino acid starvation in Escherichia coli. Molecular Microbiology. https://doi.org/10.1111/j.1365-2958.2009.06767.x
