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Cited by 74Toxoplasma gondii Research StudiesCoccidia and coccidiosis researchParasitic infections in humans and animals

The DOI matches its Crossref record.

GRA7 provides protective immunity in cocktail DNA vaccines against Toxoplasma gondii

JONGERT, E., DE CRAEYE, S., DEWIT, J., HUYGEN, K.·2007·Parasite Immunology
DOI 10.1111/j.1365-3024.2007.00961.x
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Abstract

SUMMARY: In a previous study, single‐gene vaccination with GRA1, GRA7 or ROP2 was shown to elicit partial protection against Toxoplasma gondii. In this study, the contribution of each antigen in the evoked humoral and cellular immune responses was evaluated after vaccination with plasmid mixtures containing GRA1, GRA7 and ROP2. Cocktail DNA vaccinated mice developed high antibody titers against the antigens from two‐gene DNA vaccine cocktails, but lower titres when immunized with the three‐gene cocktail. High numbers of IFN‐γ secreting splenocytes were generated predominantly against GRA7. Brain cyst burden was reduced by 81% in mice vaccinated with the three‐gene mixture and they were completely protected against acute toxoplasmosis. Similar high levels of brain cyst reductions were obtained after vaccination with cocktails composed of GRA1 and GRA7 (89% reduction), or GRA7 and ROP2 (79% reduction), but not with the cocktail composed of GRA1 and ROP2. In low dose single‐gene vaccinations, IFN‐γ and strong protection could only be elicited by GRA7. Hence, the presence of GRA7 in the DNA vaccine formulation was important for optimal protection and this was correlated with GRA7‐specific IFN‐γ production. We propose GRA7 as a main component in cocktail DNA vaccines for vaccination against T. gondii.

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Metadata

Authors
JONGERT, E., DE CRAEYE, S., DEWIT, J., & HUYGEN, K.
Affiliations
Institut Scientifique de Santé Publique (BE)
Corresponding author
JONGERT, E.
ORCID
HUYGEN, K.
Venue
Parasite Immunology
Publisher
Wiley
Published
2007-08-23
Volume
29
Issue
9
Pages
445-453
ISSN
  • 0141-9838 (ISSN-L)
  • 1365-3024
DOI
10.1111/j.1365-3024.2007.00961.x
OpenAlex
W2019087904
Cited by
74
References
0
Citations by year
2026: 1 · 2025: 1 · 2024: 7 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 4 · 2015: 3 · 2014: 5 · 2013: 5 · 2012: 5
FWCI
5.75
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Parasite Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Fonds Wetenschappelijk Onderzoek
  • Vlaamse regering
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Toxoplasma gondii Research Studies
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Coccidia and coccidiosis research
  • Life Sciences › Immunology and Microbiology › Parasitology › Parasitic infections in humans and animals
Keywords
Toxoplasma gondii, GRA7, GRA1, ROP2, protective immunity, interferon-gamma, humoral immune response, cell-mediated immunity, brain cyst burden, acute toxoplasmosis
MeSH terms
Animals, Antibodies, Protozoan, Antigens, Protozoan, Female, Interferon-gamma, Membrane Proteins, Mice, Inbred C3H, Toxoplasma, Toxoplasmosis, Animal, Protozoan Proteins, Protozoan Vaccines, Vaccines, DNA, Mice

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