Increased C availability at elevated carbon dioxide concentration improves N assimilation in a legume

Abstract

Plant growth is typically stimulated at elevated carbon dioxide concentration ([CO 2]), but a sustained and maximal stimulation of growth requires acquisition of additional N in proportion to the additional C fixed at elevated [CO 2]. We hypothesized that legumes would be able to avoid N limitation at elevated [CO 2]. Soybean was grown without N fertilizer from germination to final senescence at elevated [CO 2] over two growing seasons under fully open‐air conditions, providing a model legume system. Measurements of photosynthesis and foliar carbohydrate content showed that plants growing at elevated [CO 2] had a c. 25% increase in the daily integral of photosynthesis and c. 58% increase in foliar carbohydrate content, suggesting that plants at elevated [CO 2] had a surplus of photosynthate. Soybeans had a low leaf N content at the beginning of the season, which was a further c. 17% lower at elevated [CO 2]. In the middle of the season, ureide, total amino acid and N content increased markedly, and the effect of elevated [CO 2] on leaf N content disappeared. Analysis of individual amino acid levels supported the conclusion that plants at elevated [CO 2] overcame an early‐season N limitation. These soybean plants showed a c. 16% increase in dry mass at final harvest and showed no significant effect of elevated [CO 2] on leaf N, protein or total amino acid content in the latter part of the season. One possible explanation for these findings is that N fixation had increased, and that these plants had acclimated to the increased N demand at elevated [CO 2].