The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Two‐hybrid protein–protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors
Abstract
Summary: In vivo protein–protein interactions are frequently studied by means of yeast two‐hybrid analysis. However, interactions detected in yeast might differ considerably in the plant system. Based on GAL4 DNA‐binding (BD) and activation domains (AD) we established an Arabidopsis protoplast two‐hybrid (P2H) system. The use of Gateway®‐compatible vectors enables the high‐throughput screening of protein–protein interactions in plant cells. The efficiency of the system was tested by examining the homo‐ and heterodimerization properties of basic leucine zipper (bZIP) transcription factors. A comprehensive heterodimerization matrix of Arabidopsis thaliana group C and group S bZIP transcription factors was generated by comparing the results of yeast and protoplast two‐hybrid experiments. Surprisingly, almost no homodimerization but rather specific and selective heterodimerization was detected. Heterodimers were preferentially formed between group C members (AtbZIP9, ‐10, ‐25, ‐63) and members of group S1 (AtbZIP1, ‐2, ‐11, ‐44, ‐53). In addition, significant but low‐affinity interactions were detected inside group S1, S2 or C AtbZIPs, respectively. As a quantitative approach, P2H identified weak heterodimerization events which were not detected in the yeast system. Thus, in addition to cell biological techniques, P2H is a valuable tool for studying protein–protein interaction in living plant cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The Leucine Zipper: A Hypothetical Structure Common to a New Class of DNA Binding Proteins
Landschulz, W. H., Johnson, P. F., & McKnight, S. L. (1988) · Science · doi:10.1126/science.3289117 · Cited by 3,443
Transcriptional coactivator complexes.
Näär, A. M., Lemon, B. D., & Tjian, R. (2001) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.70.1.475 · Cited by 571
Cited by
Sugar signals and molecular networks controlling plant growth
Smeekens, S., Ma, J., Hanson, J., & Rolland, F. (2010) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2009.12.002 · Cited by 655
Self-citationLiao, Y., Zou, H.-F., Wei, W., Hao, Y.-J., Tian, A.-G., Huang, J., Liu, Y.-F., Zhang, J.-S., & Chen, S.-Y. (2008) · Planta · doi:10.1007/s00425-008-0731-3 · Cited by 398
The role of sugar signaling in plant defense responses against fungal pathogens
Morkunas, I., & Ratajczak, L. (2014) · Acta Physiologiae Plantarum · doi:10.1007/s11738-014-1559-z · Cited by 360
The heat-stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses
Huang, Y.-C., Niu, C.-Y., Yang, C.-R., & Jinn, T.-L. (2016) · Plant Physiology · doi:10.1104/pp.16.00860 · Cited by 320
Broeckx, T., Hulsmans, S., & Rolland, F. (2016) · Journal of Experimental Botany · doi:10.1093/jxb/erw416 · Cited by 267
Metadata
- Authors
- Ehlert, A., Weltmeier, F., Wang, X., Mayer, C. S., Smeekens, S., Vicente‐Carbajosa, J., & Dröge‐Laser, W.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2006-04-21
- Volume
- 46
- Issue
- 5
- Pages
- 890-900
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2090412780
- Cited by
- 252
- References
- 2
- FWCI
- 16.65
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Two‐hybrid protein–protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors. https://app.cochat.ai/papers/10.1111/j.1365-313x.2006.02731.x
- The paper
- Ehlert, A., Weltmeier, F., Wang, X., Mayer, C. S., Smeekens, S., Vicente‐Carbajosa, J., & Dröge‐Laser, W. (2006). Two‐hybrid protein–protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors. The Plant Journal. https://doi.org/10.1111/j.1365-313x.2006.02731.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
