The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
MicroRNA399 is a long‐distance signal for the regulation of plant phosphate homeostasis
Open accessAbstract
Summary: The presence of microRNA species in plant phloem sap suggests potential signaling roles by long‐distance regulation of gene expression. Proof for such a role for a phloem‐mobile microRNA is lacking. Here we show that phosphate (Pi) starvation‐induced microRNA399 (miR399) is present in the phloem sap of two diverse plant species, rapeseed and pumpkin, and levels are strongly and specifically increased in phloem sap during Pi deprivation. By performing micro‐grafting experiments using Arabidopsis, we further show that chimeric plants constitutively over‐expressing miR399 in the shoot accumulate mature miR399 species to very high levels in their wild‐type roots, while corresponding primary transcripts are virtually absent in roots, demonstrating shoot‐to‐root transport. The chimeric plants exhibit (i) down‐regulation of the miR399 target transcript ( PHO2 ), which encodes a critical component for maintenance of Pi homeostasis, in the wild‐type root, and (ii) Pi accumulation in the shoot, which is the phenotype of pho2 mutants, miR399 over‐expressers or chimeric plants with a genetic knock‐out of PHO2 in the root. Hence the transported miR399 molecules retain biological activity. This is a demonstration of systemic control of a biological process, i.e. maintenance of plant Pi homeostasis, by a phloem‐mobile microRNA.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Mineral Nutrition of Higher Plants
(1995) · Elsevier eBooks · doi:10.1016/b978-0-12-473542-2.x5000-7 · Cited by 19,181
Real-time quantification of microRNAs by stem-loop RT-PCR
Chen, C. (2005) · Nucleic Acids Research · doi:10.1093/nar/gni178 · Cited by 4,952
Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
Ramakers, C., Ruijter, J. M., Deprez, R. H. L., & Moorman, A. F. M. (2003) · Neuroscience Letters · doi:10.1016/s0304-3940(02)01423-4 · Cited by 3,709
Czechowski, T., Stitt, M., Altmann, T., Udvardi, M. K., & Scheible, W.-R. (2005) · Plant Physiology · doi:10.1104/pp.105.063743 · Cited by 3,203
Self-citationPurification and Characterization
(2006) · Biomacromolecules · doi:10.1002/0470080124.ch3 · Cited by 2,309
microRNA-Directed Phasing during Trans-Acting siRNA Biogenesis in Plants
Allen, E., Xie, Z., Gustafson, A. M., & Carrington, J. C. (2005) · Cell · doi:10.1016/j.cell.2005.04.004 · Cited by 2,268
Root Architecture and Plant Productivity
Lynch, J. (1995) · Plant Physiology · doi:10.1104/pp.109.1.7 · Cited by 1,905
Raghothama, K. G. (1999) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.50.1.665 · Cited by 1,724
Raghothama, K. G., & Karthikeyan, A. S. (2005) · Plant and Soil · doi:10.1007/s11104-004-2005-6 · Cited by 1,640
PHO2, MicroRNA399, and PHR1 Define a Phosphate-Signaling Pathway in Plants
Bari, R., Datt Pant, B., Stitt, M., & Scheible, W.-R. (2006) · Plant Physiology · doi:10.1104/pp.106.079707 · Cited by 1,145
Self-citation
Cited by
The Plant Vascular System: Evolution, Development and Functions F
Lucas, W. J., Groover, A., Lichtenberger, R., Furuta, K., Yadav, S., Helariutta, Y., He, X., Fukuda, H., Kang, J., Brady, S. M., et al. (2013) · Journal of Integrative Plant Biology · doi:10.1111/jipb.12041 · Cited by 772
The zinc homeostasis network of land plants
Sinclair, S. A., & Krämer, U. (2012) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2012.05.016 · Cited by 502
Molecular mechanisms underlying phosphate sensing, signaling, and adaptation in plants
Zhang, Z., Liao, H., & Lucas, W. J. (2014) · Journal of Integrative Plant Biology · doi:10.1111/jipb.12163 · Cited by 441
Plant Noncoding RNAs: Hidden Players in Development and Stress Responses
Yu, Y., Zhang, Y., Chen, X., & Chen, Y. (2019) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100818-125218 · Cited by 396
Chitwood, D. H., & Timmermans, M. C. P. (2010) · Nature · doi:10.1038/nature09351 · Cited by 262
Metadata
- Authors
- Pant, B. D., Buhtz, A., Kehr, J., & Scheible, W.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2007-11-06
- Volume
- 53
- Issue
- 5
- Pages
- 731-738
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2170246161
- Cited by
- 771
- References
- 46
- FWCI
- 41.58
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: MicroRNA399 is a long‐distance signal for the regulation of plant phosphate homeostasis. https://app.cochat.ai/papers/10.1111/j.1365-313x.2007.03363.x
- The paper
- Pant, B. D., Buhtz, A., Kehr, J., & Scheible, W. (2008). MicroRNA399 is a long‐distance signal for the regulation of plant phosphate homeostasis. The Plant Journal. https://doi.org/10.1111/j.1365-313x.2007.03363.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
