The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Purification and Further Properties of Single‐Strand‐Specific Nuclease from Aspergillus oryzae
Abstract
A simple scheme has been devised for the purification of Aspergillus nuclease S 1 from commercially available crude amylase powder. Five purification steps yield 90% pure nuclease with an overall yield of 27%. The enzyme is shown to be absolutely specific for single‐stranded nucleic acids. It degrades both DNA and RNA. The nuclease exhibits a sharp pH optimum at pH 4.2 and is active in low concentrations of dodecylsulfate. Aspergillus nuclease S 1 is a metalloprotein of molecular weight 32000. The utility of this nuclease for measuring annealing of labeled DNA is discussed.
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Citations
Cites
Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of Rous Sarcoma Virus
TEMIN, H. M., & MIZUTANI, S. (1970) · Nature · doi:10.1038/2261211a0 · Cited by 2,128
Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of RNA Tumour Viruses
BALTIMORE, D. (1970) · Nature · doi:10.1038/2261209a0 · Cited by 2,068
In vitro Synthesis of DNA Complementary to Rabbit Reticulocyte 10S RNA
VERMA, I. M., TEMPLE, G. F., FAN, H., & BALTIMORE, D. (1972) · Nature New Biology · doi:10.1038/newbio235163a0 · Cited by 237
Cited by
Mutagenesis at a specific position in a DNA sequence.
Hutchison, C. A., Phillips, S., Edgell, M. H., Gillam, S., Jahnke, P., & Smith, M. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)46967-6 · Cited by 522
Organization, Transcription, and Regulation in the Animal Genome
Davidson, E. H., & Britten, R. J. (1973) · The Quarterly Review of Biology · doi:10.1086/407817 · Cited by 451
Enzymatic in vitro synthesis of globin genes
Efstratiadis, A., Kafatos, F. C., Maxam, A. M., & Maniatis, T. (1976) · Cell · doi:10.1016/0092-8674(76)90027-1 · Cited by 342
Dave, V. P., Ngo, T. A., Pernestig, A.-K., Tilevik, D., Kant, K., Nguyen, T., Wolff, A., & Bang, D. D. (2019) · Laboratory Investigation · doi:10.1038/s41374-018-0143-3 · Cited by 248
Straus, S. E., Sebring, E. D., & Rose, J. A. (1976) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.73.3.742 · Cited by 194
Metadata
- Authors
- Vogt, V. M.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1973-02
- Volume
- 33
- Issue
- 1
- Pages
- 192-200
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W1996419255
- Cited by
- 859
- References
- 3
- FWCI
- 92.99
- Type
- journal article
- Language
- en
- Open access
- closed
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- Vogt, V. M. (1973). Purification and Further Properties of Single‐Strand‐Specific Nuclease from Aspergillus oryzae. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1973.tb02669.x
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